Studied The Propagated System Of Stewartia Sinensis

Stewartia sinensis is a defoliate little arbor of Theaceae,which is endangered species and rare wild ornamental tree.Studying the propagated system and applying them to the urban greening have important theory and practice meaning for protecting this species effective in another place and enriching the kinds and views of ornamental plants.This paper studied the propagated system of Stewartia sinensis systematically from the three aspects of seeding,cuttage and tissue culture,the results showed that:1,The appropriate storage way for Stewartia sinensis was wet storage,the vitality of dry storage seeds were lower than the wet storage,which were 63.3%. Moreover,the germination percentage of these seeds was 0,no matter in the laboratory or at the nursery land,while the vitality of wet storage seeds was 78.3%.In the laboratory,the absolute germination percentage was 10.2%and the average germination percentage was 5.5%,while the germination percentage of nursery land was 1%.2,The survival rate of annual branches was 0,while the biennial branches was 2.2%for ripe wood cuttings of Stewartia sinensis.3,In the ripe wood cuttings of sand storage accelerating germination of Stewartia sinensis,the highest survival rate of cuttings was processed by IBA which was 31.3%, while the rate was 15%processed by NAA and the rate was 16.7%used root inducing powder.What's more,the survival rate of dipping in high concentration of plant hormone was higher than immersed in low concentration.4,The best time for green wood cuttage of Stewartia sinensis was in first third of June,immersing the cuttings in IBA of 50ppm for an hour and rooting them in perlites could get the highest survival rate,which was 28.3%.Moreover,the survival rate was 0 in August.5,Different type and concentration of plant hormone had bigger influence to the survival rate of green wood cuttings,the highest survival rate was 28.3%and the lowest was 8.7%processed by IBA,while using NAA the highest was 18.3%and the lowest was 3.3%.The survival rate of the cuttings processed by IBA is higher than NAA no matter low or high concentration which the highest was 10%and the average was 6.7%.The survival rate of cuttings was only 1.7%when didn't use any plant hormone.6,In the sterilization process of leaves and stems of tissue culture process,the soak period of mercury bichloride which the concentration was 0.1%played an important role.The best soaking time for leaves was 7 minutes and the sterility rate could reach 69.44%,while the best time for stems was 10 minutes and the sterility rate could reach 72.22%.In the sterilization process of roots,the concentration of mercury bichloride played an important role,immersing roots in the mercury bichloride which concentration was 0.2%for 10 minutes could get a good effect and the sterility rate could reach 69.44%.7,In the experiment of tissue culture,only the leaves induced calluses successfully,and the calluses grew well among young leaves,stems and young root segments as explants.8,The best medium for inducing calluses of leaves was MS with 2.0mg/L BA and 1.0mg/L 2,4-D and 8g/L agar and 30g/L sugar and 0.5 mg/L PVP.Through this could get the highest inductivity which was 66.67%.9,In the subculture,Stewartia sinensis had fast proliferation rate,the calluses from 4 masses to 260 masses via the medium which was MS with 2.0mg/L BA and 1.0mg/L 2,4-D and 8g/L agar and 30g/L sugar and 0.5 mg/L PVP after five-month, they grew 65 times.10,Induced the adventitious buds from calluses unsuccessfully although used 6 different formulations through the bud initiation experiments for four-month..