Thtipje Expression Of P53and P21^waf1/cip1 On Retinas Of Diabetic Rats

ObjectivesTo investigate the expression of p53and p21^WAF1/CIP1in diabetes Spragne-Dawley rat’s eyes,as well as the expression correlation of both.Methods40healthy male SD rats were randomly divided into2groups:the intoexp-erimental group（20）,the control group（20）.Intraperitoneal injection60mg/kg strepto-zocin（STZ）to induce DM model,the control group conventional breeding.Use immunohistochemistry and ELISA detect the rats’ eyes respectively.Results1、The concentration measurement of p53and p21^WAF1/CIP1of retina: concen-tration level of p53and p21^WAF1/CIP1in the intoexperimental group were higher thanthe control group （P<0.05） in the retinal tissue.2、Immunohistochemistry result: The positive cell of p53and p21^WAF1/CIP1express in the retinal ganglion cell layer.The control group rat’s retina express traceamount of p53and p21^WAF1/CIP1 postive cell.The intoexperimental group’sexpression significantly increase,compared with normal control group was statistic-ally significant （P<0.05）.3、 p53and p21^WAF1/CIP1expression in the retinal tissue of diabetic SD rats werepositively correlated （rs=0.674）.Conclusion1、There is a certain link between p53、p21^WAF1/CIP1and diabetic retinopathy（diabeticretinopathy, DR）, the duration of DR longer,the expression level of bothincrease.2、p53and p21^WAF1/CIP1may participate in DR’s occurrence and development,the expression of both can be used to evaluate the the DR’s prognosis and pathological stage, which also provides the DR diagnosis, gene therapy a new idea.