Effects Of Dextran Sulfate On Expression Of Integrin Betal,VEGF And MVD, And On Treatment Of Human Gastric Cancer Cell Intraperitoneal Implantation Metastasis

Objective To observe the effect of dextran sulfate(DS) on theexpression of integrin betal,vascular endothelial growth factor (VEGF) andmicrovessel density(MVD), and on treatment implanting metastasis of gastriccancer cells in the abdominal cavity, to investigate DS on the treatment andmechanism of implanting metastasis of gastric cancer cells in the abdominalcavity.Methods The nude mice80were randomly divided into control group(30nude mice) and experimental group (50nude mice). The all nude mice wereinjected cancer cells suspension into abdominal cavity by the way of0.2ml/onlywith the number of1x107cells for establishing animal models. After24hours,the nude mice in the control group were injected dextran70into abdominalcavity by the way of1ml/only as the negative control. The nude mice inexperimental group were injected DS into abdominal cavity by the way of1ml/only. The control group and the experimental group were randomly dividedinto five groups,the nude mice were killed on the2st day after injecting gastriccancer cells, the3rd day, the7th day, and the14th day respectively, and naturaldeath after, gross exploration and HE staining to observe implantingmetastasis of gastric cancer cells in the abdominal cavity. The omentums were collected. Then the immunohistochemical method was applied to detect omentalmetastatic carcinoma integrin betal, VEGF and CD34expression, and RT-PCRmethod to detect integrin betal mRNA and VEGF mRNA expression.Results with the extension of the implanting time of gastric cancer cells,in control group and experimental group,the number of mean tumor nodulesgradually increased, and Integrin betal and VEGF expression both graduallyincreased. The number of mean intraperitoneal metastatic nodules of DSexperimental group was significantly less than that of control group P<0.001.By immunohistochemical analysis, the integrin betal, VEGF protein and MVDvalue of DS experimental group were significantly less than those of controlgroup in omentum,all P<0.001. By RT-PCR analysis, the integrin betal mRNAand VEGF mRNA of DS experimental group were significantly less than thoseof control group in omentum,both P<0.001.in the control group and theexperimental group,they are both positive correlation between integrin betaland VEGF expression in gastric cancer tissue, in the control group and theexperimental group,they are both positive correlation between VEGFexpression and MVD value in gastric cancer tissue. The survival rate of DSexperimental group was significantly longer than that of the control group,P<0.001.Conclusion Firstly, DS inhibits implanting metastasis of gastric cancercells in the abdominal cavity,and the survival of DS experimental group wassignificantly prolongedand,and was associated with decreased expression ofintegrin betal,VEGF and MVD value. Secondly, there is a positive correlationbetween integrin betal expression and VEGF expression in gastric cancer tissueof omentum. Thirdly, DS inhibits implanting metastasis of gastric cancer cells,Possibly by downregulating the expression of integrin betal and VEGF expression. Expression of integrin betal and VEGF was declined, which may beone of the mechanisms of DS treating implanting metastasis of gastric cancercells.