Expression And Significance Of NF-kappa B And Related Factors In Cervical Cancer Cell Lines

Cervical cancer is a global disease. It’s incidence rate occupies the second woman malignant tumor, especially in developing country. Should sickness cause of disease not yet completely understand, shift way mainly for direct spread and lymph shift. The HPV infection is cervical cancers primary hazard factor, therefore may think initially cervical cancer is the inflammation relevant tumor. NF-κB is in the cell in the signal transmission process the most important nucleus transcription factor, and it is also playing the extremely vital role in the inflammation to cancer’s progress. It may with many cell factors, the inflammation hasten gene and so on factor, adherency member starts or increases the hadron specificity union, starts and adjusts these genes the expression and the duplication, thus lies between leads the immune response, the inflammation to respond, cancer progress and the cell multiplication, the adherency and perish weakly process. This topic-based group earlier period’s findings already indicated that the white blood cell lies between the element8(IL-8), in has the lymph node to shift cervical cancer to organize the expression not to have lymph node shift cervical cancer organization high3.4times,extrapolates the two possibly in cervical cancer’s occurrence, the development the influential role.Purpose:Research NF-κB, IL-8in person cervix of the uterus cancer cell bead Siha, Caski expression as well as CXCR2, BCL-2, VEGF, MMP2and so on multiple coefficient of correlation in person cervix of the uterus cancer cell bead Caski expression. Thus explores them in the cervix of the uterus carcinogenesis development function, provides the new mentality and the method take the time further thorough understanding cervical cancers pathogenesis and as the time diagnosis.Methods:First, uses the immunocytochemistry, method examination cervix of the uterus cancer cell bead Siha, Caski and so on Western Blot, ELISA NF-κB, the IL-8 expression; Next, the law examines in the Caski cell bead CXCR2, BCL-2, VEGF, MMP2and so on multiple coefficient of correlation expression, and (MTT) observes cervix of the uterus cancer cell bead Caski using the cell multiplication experiment to stimulate the before and after multiplication situation in NF-κB inhibitor PDTC, and selects the most effective concentration and the time. Finally, stimulates cervix of the uterus cancer cell bead Caski with PDTC, and through immunocytochemistry, method examination NF-κB, IL-8, CXCR2, Bcl-2, VEGF, MMP2and so on Western Blot, ELISA and so on multiple coefficient of correlation expression.Result:NF-κB, IL-8in person cervix of the uterus cancer cell bead Siha, Caski have the masculine gender to express, and is higher than in Siha in the high shift cell line Caski expression the expression. The two compare the difference to have the significance (P<0.05). PDTC may suppress cervix of the uterus cancer cell bead Caski obviously the multiplication activeness, and suppresses NF-κB, IL-8, CXCR2, Bcl-2, VEGF, the MMP2expression.Conclusion:NF-κB, IL-8are participate in the inflammation and the tumor have the development important cell factor. The two were higher than in Siha in the Caski expression the expression also fully to show the two obviously and cervical cancer’s progress close related. NF-κB is a politropism important transcription factor. It may unify in some target gene start sub-and increases the hadron (for instance, MMP2, IL-8, VEGF, Bcl-2and so on). But these control the cancer cell the attack, shift and blood vessel occurrence. This indicated that the NF-κB indirect control inflammation, the cell are perishing weakly as well as cancer cell’s attack and the shift.Obviously NF-κB in cervical cancer’s occurrence development very important. Will be helpful in view of the NF-κB research to further understood that cervical cancer’s occurrence mechanism, provides the theory basis for cervical cancer’s clinical diagnosis, seeks for a new mentality for cervical cancer’s personalized treatment.