Related Study Between CXCL12and Unexplained Recurrent Spontaneous Abortion

Objective:By measuring early pregnant women with history of habitual abortion and normal early pregnant women between the villi and decidua chemotactic factor12(CXCL12) expression, to explore CXCL12and recurrent spontaneous abortion, to understand CXCL12in pregnancy role.Methods:Using immunohistochemical SABC were detected CXCL12expression in fresh villi and decidua group of30case studies (RSA group) and control group (normal pregnancy group) two sets of early pregnancy women artificial abortion after termination of pregnancy case. Using the HPIAS-1000high resolution color pathological image analysis system for processing. For each slice (at a magnification of400times) were randomly taken10non-overlapping field of view, average gray luminosity of each of its field of view is rendered positive expression were measured value A, A lower value if the expression of the stronger, and vice versa is no different. The experimental data obtained using SPSS13.0statistical software for statistical analysis, data expressed as mean±standard deviation (), between the two groups were compared using the t-test, P<0.05as statistically significant difference.Results:1.CXCL12expression and distribution of groups villi:URSA group (study group) and normal pregnancy group (control group) can be seen more complete trimester chorionic villi structure. Villi syncytiotrophoblast layer and cytotrophoblast and loose in the middle of stromal part. The outer layer of the cell to fit trophoblastic layer, the more abundant cytoplasm, rendered as ground glass. The trophoblastic layer inner cell boundary more clearly, nuclear round, was a small bubble. Stromal cells were mostly stellate-shaped, visible small blood vessels in the interstitium. The CXCL12mainly expressed in the the trophoblastic villi cytoplasm, and cell trophoblast cells compared two groups of villi syncytiotrophoblast than strong positive staining of the cytoplasm of cells, interstitial slight coloring Ursa group trophoblastic cytoplasm weak positive staining than normal pregnancy group (see Figure1,2). The URSA group villi CXCL12expression was significantly lower than the normal pregnancy group (P<0.05), the difference was statistically significant. Table2.2. CXCL12expression and distributionof groups decidua:two sets of decidua by decidual cells, glands and decidual stromal vascular composition. Decidual cells volume is relatively large, mostly polygonal or circular, its abundant cytoplasm, the nucleus is relatively narrow. Cell gap increases. Decidua glands with papillary structures, and was highly secretory state, the cytoplasm of epithelial cells rich. Decidual stroma can be seen in small blood vessels. CXCL12is mainly expressed in the decidual cells, the cytoplasm of epithelial cells and vascular endothelial cells, a strong positive staining of decidual cells (3,4). The URSA group decidua CXCL12expression was significantly lower than the normal pregnancy group (P<0.05), the difference was statistically significant. Table3.Conclusion:The high expression of chemokine12(CXCL12) in the villi and decidua of early pregnancy maternal-fetal interface showing a state plays an extremely important role in the maintenance of the whole pregnancy process Expressions of chemokine CXCL12expression decreased with unexplained recurrent spontaneous abortion having a certain correlation.