The Expression And Clinical Significance Of MMP-9、MMP-14in Osteoarthritis Patients Synovium Of Knee Joint

Objective：Osteoarthritis is a kind of chronic degenerative disease pathological change in this disease are consist of thedestroy of integrality of the joint disease, its pathogenesis is not clear until now. In recent years, with thedevelopment of molecular biology, more and more domestic and foreign scholars are beginning to researchthe relationship of matrix metalloproteinase and OA. This experiment was used to study the MMP-9/-14expression and correlation in different stages of OA and normal synovium, to elucidate the significance ofMMP-9/-14in the pathogenesis of OA and provide a theoretical basis to find effective interventions targetfor the clinical treatment of OA.Methods：Eighty cases synoviums are from the patients in department of orthopedics of the first affiliated hospital ofShi He-zi University from the February2011to the August2012,which operation include the knee joint ofarthroscopy, arthroplasty, amputation and so on. All of the patients were diagnosed by the doctor oforthopedics or using the proposed criteria from American in1995.The collected synovium were washedthree times with saline in one hour after operation, then cut by half synovium into liquid nitrogen, the otherfixed by10%paraformaldehyde. Finally, Immunohistochemically and Western blot were used to detect theexpression of MMP-9/-14protein in eighty cases of osteoarthritis. The results according to SPSS17.0statistical analysis software: All of the categorical data is signed χ2-test, and the mean will adopt mean士standard deviation, and the mean is different, two groups compared with T-test, use correlation coefficientanalysis between MMP-9and MMP-14,according to P＜0.05presents statistics different.Results：The expression of MMP-9and MMP-14protein in osteoarthritis patients synovium of knee joint wasmarkedly higher that in the normal synovium（χ2=18.62/29.46，P＜0.01）, and the rownish yellow wasmainly located in the cytoplasm. In OA group, Pearson correlation coefficient between MMP-9andMMP-14is0.999（r=0.999，P＜0.01）, the expression of MMP-9were positively correlated with MMP-14in OA synovium tissue. Western blot results showed that expression of MMP-9/-14protein in osteoarthritispatients synovium of knee join（0.951士0.302/1.042士0.219）was significantly higher than that in thenon-osteoarthriti（s0.275士0.134/0.256士0.05，P＜0.01）, Pearson correlation coefficient between MMP-9/-14is0.997（r=0.997，P＜0.01）and were positively correlated with MMP-9/-14protein and OA.Conclusion：In OA group, the expression of MMP-9and MMP-14protein in synovium tissue were higher than normalgroup, they were positively correlated with each other and at the same time were close relationship with OA. So they maybe the marker of diagnosis、judgment of osteoarthritis.