| ObjectiveTo investigate the expression of caveolin-1,FHIT and MMP-9in cervical cancer andcervical intraepithelial neoplasia(CIN), analyzing their expression in different cervixlesions, and their relationship with clinical and pathological features. To explore theirassociations with the carcinogenesis and development of cervical cancer and evaluate theirclinical significances.MethodsS-P immunohistochemistry was used to measure the expression of caveolin-1, FHITand MMP-9in36cases of CIN,45cases of cervical cancer and another13cases of normalcervical tissues selected from paraffin wax-embodied specimens. The36cases of CINincludes13CINâ… ,11CINâ…¡,12CINâ…¢. The45cases of cervical cancer includes15sdageâ… ,12stageâ…¡,18stageâ…¢ by FIGO stagingï¼›13G1,15G2,17G3by pathologic grading.Squamous cell cancer was41and adenacarcinoma was4. Among the45cases，24wereaccompanied with lymph node metastasis. Statistical analysis utilizing chi-square test andSpearman correlation analysis was performed with statistical program for social sciences13.0software.Results1.The positive rate of caveolin-1expression was a decreasing trend in the normalcervix tissues，CIN and cervical cancer. The reduced proportions of caveolin-1expressionwere100.00%(13/13),63.89%(23/36) and31.11%(14/45),which had significant differencesbetween any two groups(P＜0.05). The positive rate of caveolin-1expression was adecreasing trend in the tissue of CINâ… â†'CINâ…¡â†'CINⅢ，The reduced proportions ofcaveolin-1expression were84.62%(11/13),63.64%(7/11) and50.00%(6/12), But there wasno significant differences among CIN groups(P＞0.05).The expression of caveolin-1in the cervical cancer is related with the pathological grade and lymph node metastasis(P＜0.05),but the expression is no related with the age, histological type and clinical stage(P＞0.05).2.The positive rate of FHIT expression was a decreasing trend in the normal cervixtissues，CIN and cervical cancer. The reduced proportions of FHIT expression were92.31%(12/13)，58.33%(21/36) and22.22%(10/45),which had significant differencesbetween any two groups(P＜0.05).The positive rate of FHIT expression was a decreasingtrend in the tissue of CINâ… â†'CINâ…¡â†'CINâ…¢,The reduced proportions of FHITexpression were6.92%(10/13),54.55%(6/11) and41.67%(5/12),But there was nosignificant differences among CIN groups(P＞0.05).The expression of FHIT in the cervicalcancer is related with the histological type,pathological grade and lymph node metastasis(P＜0.05),but the expression is no related with the age and clinical stage(P＞0.05).3.The positive rate of MMP-9expression was an increasing trend in the normal cervixtissues,CIN and cervical cancer.The rising proportions of MMP-9expression were7.69%(1/13),52.78%(19/36) and86.67%(39/45),which had significant differences betweenany two groups(P＜0.05).The positive rate of MMP-9expression was an increasing trendin the tissue of CINâ… â†'CINâ…¡â†'CINâ…¢,The rising proportions of MMP-9expressionwere46.15%(6/13),54.55%(6/11) and66.67%(8/12),But there was no significantdifferences among CIN groups(P＞0.05).The expression of MMP-9in the cervical canceris related with the clinical stage and lymph node metastasis(P＜0.05),but the expression isno related with the age, histological type and pathological grade.(P＞0.05).4.The expressions of caveolin-1and MMP-9had negative correlation in the samesamples((rs=-0.323,P <0.05).5.The expressions of FHIT and MMP-9had negative correlation in the samesamples(rs=-0.431,P <0.05).6.The expressions of caveolin-1and FHIT had positive correlation in the samesamples((rs=0.350,P <0.05).Conclusion1.Compared with CIN and normal cervix tissues,the progressive down-regulation andabsence of caveolin-1may play an important role in the carcinogenesis,development andmetastasis of cervical cancer.It may be acted as a valuable tumor marker for judging theadvancement and prognosis of cervical cancer.2.The loss of FHIT expression is an early event and has close relations with the tumor occurance and development in cervical cancer,which might be a useful indicator inidentifying the possibility of the progression of CIN into invasive cervical cancer andcould provide a reference foundation to monitor lapse of CIN and judge prognosis ofcervical cancer.3.The over expression of MMP-9is correlated with the invasion and metastasis ofcervical cancer,The positive expression of MMP-9could be used to estimate the metastasispotentiality, predict the prognosis and help clinicians to make therapeutic strategies.4.There is the correlation among the expression of caveolin-1,FHIT and MMP-9,whichindicate they may involve in the occurrence and development of cervical lesions and playan important role in the infiltration and metastasis changes of cervical cancer.5.Detection the three genes of cervical cancer has great value to evaluate assessment ofthe biological behavior, it can help clinicians to evaluate the prognosis,provide the basis forclinical treatment and maybe become a new molecular target therapy site in treatment ofcervical cancer. |
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