The Correlations Between CYP27B1Gene Polymorphism And Schizophrenia And Metabolism Markers

Objective It has a higher metabolic syndrome in SCZ patients after drug therapy than Mets, and the mechanisms of the SCZ remain unclear. The aim of the present study was to examine the potential association between CYP27B1SNPs (rs10877012and rs4646536) and the risk of SCZ and the risperidone-induced alteration of the metabolic parameters.Methods:222hospitalized patients with schizophrenia and150healthy volunteers were enrolled. The target regions containing the polymorphisms of DNA were amplified by polymerase chain reaction (PCR) followed by ligation detection reaction (LDR) as previous reported. The measurement of serum Ins concentrations was performed on the ADVIA Centaur automated chemiluminescence immunoassay analyzer (Bayer Diagnostics, Tarrytown, USA). Total serum cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels of the patients were analyzed by the enzymatic procedures applying the Boehringer Mannheim/Hitachi717automated chemistry analyzer (Boehringer Mannheim Hitachi, Tokyo, Japan). All the data was analyzied by using SPSS16.0software.Results:In this study, all the SNPs conformed to the Hardy-Weinberg equilibrium. There were no significant differences between schizophrenic and control subjects in the distribution of alleles or genotypes of the two SNPSs. The TG and insulin levels significantly increased after4weeks risperidone treatment (P<0.05), the status of TC, HDLC and LDLC were unchanged. ANOVA indicates that a significant difference of Ins levels was found among the different genotypes of rs10877012and rs4646536in patients with SCZ. Our data indicates that the two SNPs may associated with the onset of drug-induced insulin resistance.Conclusion:Collectively, we have observed no association between SCZ and two SNPs of the CYP27B1gene. The SCZ patients with risperidone mono therapy have significant increase of insulin and TG level in week4comparing to week0. Specifically, rs10877012genotype have association with the changes of insulin levels in week4and week0, and this genotype Patients with T gene is lean to have a high insulin level after risperidone treatment. rs4646536genotype is associated with changes of insulin levels only in week4and week0, and these genotype Patients with C gene is trend to have high insulin level. These data suggest that CYP27B1is not a major gene for SCZ in Chinese Han population. However, our study provides evidence that SNPs in the CYP27B1might be associated with the susceptibility to the insulin resistance in risperidone-treated patients.