The Expression And Clinical Significance Of MACC1and C-Met In Cervical Carcinoma

Objective:This study is exploring the expression of proteins and mRNAs of metastasis-associated in colon cancer-1(MACC1), hepatocyte growth factor receptor (c-Met) in normal cervical tissue, moderate-severe cervical intraepithelial neoplasia, cervical carcinoma and then investigating the relationship with clinical pathological features of cervical carcinoma.Method:1. The immunohistochemical staining streptavidin-preosidase method was applied to detect the expression of MACC1and c-Met protein in10normal cervical samples,17CINⅡ-Ⅲ samples,91cervical carcinoma samples, then analyzing the correlation the protein expression of MACC1and c-Met in cervical carcinoma.2. The real-time fluorescent quantitative PCR technology was used to detect the expression of MACC1and c-Met mRNA in10normal cervical samples,10CINⅡ-Ⅲ samples and41cervical carcinoma samples. Then analyzing the correlation the mRNA expression of MACC1and c-Met in cervical carcinoma.3. Analyze the relationship between the expression of MACC1/c-Met with the clinical and pathological factors of cervical carcinoma.4. Analyze the relationship between the MACC/c-Met protein expression level and pelvic lymph node metastasis in cervical carcinoma.5. The data was analyzed with the statistics software SPSS17.0and set inspection level a at0.05.Results:1. The positive expression rates of MACC1protein, c-Met protein in normal cervical, CINⅡ-Ⅲ and cervical carcinoma were0.0%,76.5%,83.5%;10%,70.6%,80.2%respectively. The differences between the three groups have statistics significance. The positive expression rates of MACC1protein, c-Met protein in cervical carcinoma, CINⅡ-Ⅲ were significantly higher than that in normal cervical tissue, there were statistical significance. The strong positive expression rates of MACC1protein, c-Met protein in cervical carcinoma were significantly higher than that in CINⅡ-Ⅲ tissue, there were statistical significance. There was a positive correlation between MACC1protein level and c-Met protein level in cervical carcinoma.2. The expression of MACC1protein in cervical carcinoma was significantly associated with clinical stage, histological differentiation degrees, high risk HPV infection, pelvic lymph node metastasis, myometrium invasion depth (P<0.05), however age, clinical stage, histological type, vascular invasion depth and tumor diameter did not show strong association(P>0.05).3. The expression of c-Met protein in cervical carcinoma was significantly associated with histological differentiation degrees, high risk HPV infection, pelvic lymph node metastasis, myometrium invasion depth (P<0.05), however not associated with ages, clinical stage, histological type, vascular invasion, and tumor diameter (P>0.05).4. The single factor comparative analysis between cervical carcinoma pelvic lymph node metastasis group and no cervical carcinoma pelvic lymph node metastasis group demonstrates the expression of MACC1protein and c-Met protein, histological differentiation degrees, vascular invasion, muscle invasion depth were associated with cervical carcinoma pelvic lymph node metastasis (p<0.05), and further logistic regression analysis shows that MACC1protein level and depth of muscle invasion were independent risk factors of cervical carcinoma pelvic lymph node metastasis (p<0.05).5. The expression levels of MACC1mRNA and c-Met mRNA in normal cervical tissue, CINⅡ-Ⅲ and cervical carcinoma were1.172±0.684,2.950±1.366,14.378±9.652;2.535±2.146,7.103±3.763,28.135±15.418respectively. The difference between the three groups have statics significance (P=0.000<0.05). There was positive correlation between MACC1mRNA level and c-Met mRNA level in cervical carcinoma (r=0.648, P=0.000<0.05).6. The expression of MACC1mRNA and c-Met mRNA in cervical carcinoma was significantly associated with histological differentiation degrees, pelvic lymph node metastasis, muscle invasion depth (P<0.05), however not associated with age, clinical stage, histological type, high risk HPV infection, vascular invasion, and tumor diameter size (P>0.05).Conclusion:1. The expressions of MACC1protein and mRNA, c-Met protein and mRNA in cervical carcinoma, CINⅡ-Ⅲ tissue were significantly higher than that in normal cervical tissue. There was positive correlation between MACC1expression and c-Met expression in cervical carcinoma, which suggested MACC1and c-Met are associated with the occurrence and progression of cervical carcinoma.2. The expression of MACC1protein and mRNA, c-Met protein and mRNA in cervical carcinoma were associated with histological differentiation degrees, pelvic lymph node metastasis, muscle invasion depth, which suggested that MACC1and c-Met were associated with cervical carcinoma invasion and metastasis.3. MACC1protein expression level is an independent risk factor of cervical carcinoma pelvic lymph node metastasis.