To Study The Influence Of Pentoxifylline On Biological Behavior In Melanoma A375Cells

Objective To study the inhibitory effect of Pentoxifylline on A375melanoma metastasis and its mechanism.Methods The effects of pentoxifylline on A375melanoma proliferation were detected by cell viability assay and colony formation assay.The effects of pentoxifylline on A375melanoma apoptosis and cell cycles were detected by Flow cytometry. The effects of Pentoxifylline on A375melanoma metastasis and Motility by adhesion assay and wound healing assay.The effects of pentoxifylline on A375melanoma expression and activity of MMP-9and MMP-2.The effects of pentoxifylline on A375xengraft growth in vivo were detected by animal experimentResults The results implicate that PTX at sub-toxic doses exhibited an inhibitory effect on the abilities of cellular proliferation as shown by MTT and colony formation assay. It impedes migration and also induces apoptosis. A375cells pretreated with PTX showed decrease in adhesion to both matrigel and collagen type IV. Further, gelatin zymography result reveals that PTX treatment decreases the secretion of MMP2and MMP9. Finally, PTX significantly inhibited A375subcutaneous tumour xenograft growth without having any toxicity.Conclusion PTX at subtoxic doses affected melanoma metastasis at multiple steps in vitro as well as tumour growth in vivo.