Efficacy Study And Component Analysis Of Radix Polygalae Decoction Processed By Magnolia Officinalis Juice

Objective and significance:In order to facilitate clinical use and provide reference, our research group studies the method of Magnolia officinalis juice processed Radix Polygalae and choose, analyse the optimal one which can decrease the side effect and remain the original effect.Methods:To use the main method in efficacy and analysis with muLtisubjects, as follow studies.①To establish the optimal processed Radix Polygalae with orthogonal experiment by the method of semi-solid dextrin.②To confirm wether the optimal processed Radix Polygalae has an obvious effects on the pharmacodynamics of Radix Polygalae such as sedative, relieving cough and removing phlegm by the mice experiments of collaborative with pentobarbital sodium, independent activities, induced cough by concentrated ammonia, excretion of phenol red from tracheal.③To analyse the optimal processed Radix Polygalae by HPLC in tenuifolin, honokiol, magnolol and fingerprint.ResuLts:(1)①The processed Radix Polygalae5group and the12group can significantly reduce the gastric residual rate and increase the intestinal propuLsion rate (P<0.05) compared with Radix Polygalae group. Compared with the control group,there is no significant change in the processed Radix Polygalae5(P>0.05) whereas significant increase in the12group with gastric residual rate(P<0.05).(2)①In the experiment of collaborative with pentobarbital sodium, the high-dose processed Radix Polygalae5group, the middle-dose processed Radix Polygalae5group and the low-dose processed Radix Polygalae5group has the effect of sedative compared with the control group (P<0.05). In the experiment of independent activities, there is not any significant change in every group before administration. After administration, compared with the control group, the high-dose processed Radix Polygalae5group can restrain the activities in the1st d,3rd d and7th d (P<0.05, P<0.01). Compared with the control group, the middle-dose processed Radix Polygalae5group can restrain the activities in the1st d,3rd d and the Radix Polygalae group can restrain the activities in the3rd d (P<0.05).②In the experiment of induced cough by concentrated ammonia, every group can very significantly decrease the coughing degree(P<0.01) compared with the control group. And the high-dose processed Radix Polygalae5group can very significantly prolong the latency time of coughing. In the experiment of excretion of phenol red from tracheal, there is no significance of absorbance between the Magnolia officinalis disturbed group and the control group (P>0.05). Compared with the control group, the high-dose processed Radix Polygalae5group can significantly increase absorbance (P<0.05) whereas no significance in the Radix Polygalae group and the Magnolia officinalis group (P>0.05).(3)①In the experiment of content determination, the honokiol and magnolol in the processed Radix Polygalae5decrease slightly compared with Magnolia officinalis.On the other hand, the tenuifolin in the processed Radix Polygalae5increase compared with Radix Polygalae.②In the study of fingerprint, the main compositions in the processed Radix Polygalae5belong to Radix Polygalae, and compositions mainly belong to Magnolia officinalis before40min. From the peak area, there is not any change between the processed Radix Polygalae5and Radix Polygalae. Contents in Magnolia officinalis are slightly higher than the processed Radix Polygalae5.Conclusions:①The optimal Magnolia officinalis juice processed Radix Polygalae can reduce the side effect and remain the original effect of sedative, relieving cough and removing phlegm which shows the dose dependence.②In vitro experiment, there is not any change in optimal Magnolia officinalis juice processed Radix Polygalae.