| Aim:Research the effect of clerodendranthus spicatus on SD rats with chronic glomerulonephritis in C-BSA modelMethod:Select SD rats (clean grade healthy)90, male and female half, body weight was200±15g,1weeks after adaptive feeding common feed, placed in metabolic cages for urine collection, two determination of urinary protein was negative.16rats random served as normal control group (the normal group), methods74rats by modified Border method to establish rat model of chronic nephritis. Experiments were carried out for8weeks, the first week of the test were pre immune on rats, the cationic bovine serum albumin (C-BSA) and incomplete Freund’s adjuvant (FIA) mixed by1:1, mixing into a milky white suspension. Using the2.0mg of the suspension was inoculated subcutaneously in rats neck, back, groin and armpits. The normal group were injected with normal saline..In experiments second to fourth weeks, the C-BSA and FIA as1:1Hybrid, mixing into a milky white suspension. Using the2.0mg of the suspension was inoculated subcutaneously in rats neck, back, groin and armpits. Then mix the milky white suspension was injected into the tail vein of rats. For the first time in rats by tail vein injection amount is0.5mg/each, every1days of intravenous injection of1time. Injection of gradually increasing0.5mg every time when injection, Within3weeks of the injection volume delivery to2.5mg/each, the normal group were injected with normal saline. Experiment fifth to eighth weeks of treatment, the model and the survival of60rats were randomly divided into clerodendranthus spicatus water extract treatment group (the water extraction group), clerodendranthus spicatus ethanol extract treated group (the ethanol extraction group), the model of chronic nephritis group (the model group), tripterygium glycosides treatment group (the positive control group),15rats in each group. Water extraction and alcohol extraction group respectively give clerodendranthus spicatus the water extract solution6.3g/kg/d and clerodendranthus spicatus alcohol extrac aqueous solution6.3g/kg/d gastric lavage, the positive control group were given tripterygium glycosides tablet solution10g/kg/d orally, the normal group, model group were given normal saline, daily8p.m. gastric lavage for a month. In experiment4,5,6and8weeks of each measuring a24hours urine protein. In experiment4,8week each one measuring the serum urea nitrogen, creatinine and blood lipid (total cholesterol, triglyceride and low density lipoprotein cholesterol). Experiment eighth weekend, all rats were killed, get the bilateral renal, preserved in10%formalin solution. Kidney specimens after HE stained pathological sections, to observe the renal pathological changes under light microscope.Results:1.24hour urine protein: Experiment second weekend, except the normal group, other groups showed different degrees of urinary protein. Experiment fourth weekend, except the normal group, the rest groups urinary protein reached the highest value, there is significant difference compared with the normal group (P<0.05), but there was no significant difference between groups (P>0.05). Experiment fifth to eighth weeks, water extraction, ethanol extraction group and urinary protein in experimental group fourth, the positive control group compared to the weekend, have varying degrees of decline. Results eighth weeks, urine protein of rats in model group compared with the normal group, there was significant difference (P<0.05), group, ethanol extraction group and positive control group, the urine protein extraction water compared with the model group, with significant differences (P<0.05), The treatment group (group of water extraction, alcohol extraction group and positive control group) and urinary protein compared with no statistical significance (P>0.05).2.The serum urea nitrogen, creatinine (BUN, Cr):The fourth week of the test, compared with the normal group, model group, group of water extraction, ethanol extraction group and positive control group BUN, Cr had significant difference (P<0.01), model group, water extraction, alcohol extraction group, comparison is not statistically significant between the positive control group (P>0.05). The experiment of eighth weeks, compared with the normal group, model group BUN, Cr had a very significant difference (P<0.05), compared with the model group, water extraction, ethanol extraction group and positive control group BUN, Cr had a very significant difference (P<0.01), the treatment group (group of water extraction, alcohol extraction group and positive control group BUN, Cr) compared with no statistical significance (P>0.05).3. Triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C): The fourth week of the test, compared with the normal group, model group, group of water extraction, ethanol extraction group and positive control group TG, TC, LDL-C were significantly different (P<0.01), the model group, water extraction, alcohol extraction group, comparison is not statistically significant between the positive control group (P>0.05). The experiment of eighth weeks, compared with the normal group, model group, TG, TC, LDL-C were significantly different (P<0.05, P<0.01), compared with the model group, water extraction, ethanol extraction group and positive control group TG, TC, LDL-C have significant difference (P<0.05, P<0.01), the treatment group (group of water extraction, alcohol extraction group and positive control group) and urinary protein compared with no statistical significance (P>0.05).4. Pathological observation of renal tissue:Glomerular cells and renal tubular structure of normal group is clear, no interstitial infiltration of inflammatory cells, edema and fibrosis, mesangial cell proliferation and matrix free, the model group see mesangial cells and mesangial matrix proliferation and associated with mild to moderate renal small balloon adhesions, glomerular vascular and bottom die is markedly thickened, with segmental sclerosis; the treatment group (group of water extraction, alcohol extraction group and positive control group) of the glomerular wall smooth, interstitial hyperplasia was significantly reduced, with a small amount of inflammatory cell infiltration, water extraction, alcohol extraction group, positive compared to the control group, no significant difference.Conclusion:1.C-BSA models were successfully produced.2.Clerodendranthus spicatus can reduce urinary protein in C-BSA rat mode, improvement of BUN, Cr and blood lipids, the renal function of rats to protect.3. Clerodendranthus spicatus can improve the pathological lesion of renal tissue in rats C-BSA model role.4. Clerodendranthus spicatus in the treatment of C-BSA rat model and effect of tripterygium glycosides tablet equivalent. |
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