Expression Of Tumor-infiltrating Lymphocytes In Advanced Gastric Carcinoma And Its Relationship With The Prognosis Of Patients

Objective: To investigate the expression of tumor infiltrating CD4+,CD8+, PD-1+T cells in advanced gastric carcinoma tissue and its relationshipwith the prognosis of patients.Methods: Immunohistochemistry was used to detect the number and theexpression rate of tumor infiltrating CD4+, CD8+, PD-1+T cells in advancedgastric carcinoma tissue,and analyze the correlation between them and theclinical pathological characteristics and the prognosis of patients.Results:1. The number and the expression rate of CD4+, CD8+, PD-1+Tcells and the ratio of CD4+/CD8+T cells in the Cancer nests of the advancedgastric carcinoma tissue were significantly lower than those in the stroma; thedifference was statistically significant(all P<0.05).2. The expression of CD4+,CD8+, PD-1+T cells and the ratio of CD4+/CD8+T cells in advanced gastric carcinoma tissue is not related to the patient’s gender, age, tumor size, tumor siteand the degree of differentiation of tumor (all P>0.05), but is related to theTNM stage, lymph node metastasis and prognosis (all P <0.05). The positiveexpression rate of CD4and CD8and the ratio of CD4+/CD8+T cells in stage IIpatients was higher than those in stage III patients; The positive expression rateof PD-1in stage II patients was lower than those in stage III patients; Thepositive expression rate of CD4and CD8and the ratio of CD4+/CD8+T cellsin lymph node metastases group was lower than those in the group withoutlymph node metastasis; The positive expression rate of PD-1in lymph nodemetastases group was higher than those in the group without lymph nodemetastasis; But the positive expression rate of CD4and CD8and the ratio ofCD4+/CD8+T cells in≥5-yearssurvival group was higher than those in≤3-years survival group; the positive expression rate of PD-1in≥5-yearssurvivalgroup was lower than those in≤3-years survival group; the difference wasstatistically significant(all P<0.05).3. The positive expression rate of PD-1onCD8+T cells in advanced gastric carcinoma tissue is higher than those onCD4+T cells, the difference was statistically significant (P<0.05).Conclusion:1. Tumor infiltrating CD4+, CD8+, PD-1+T cells in advancedgastric carcinoma tissue are mainly distributed in the stroma, and the expressionis associated with clinical stages, lymph node metastasis and prognosis.2.Tumor infiltrating CD4+, CD8+, PD-1+T cells and the ratio of CD4+/CD8+Tcells in advanced gastric carcinoma tissue reflects the local anti-tumor immune status in patients with gastric carcinoma；CD4, CD8are positive regulator of theimmune, the increase of the number of CD4+, CD8+T cells and the ratio ofCD4+/CD8+T cells prompt good prognosis; but PD-1is a negative regulator ofimmune, mainly expresses on CD8+T cells, the increase of the number ofPD-1+T cells prompt poor prognosis.3. Tumor infiltrating CD4+, CD8+,PD-1+T cells and the ratio of CD4+/CD8+T cells in advanced gastric carcinomatissue all may be used as indicator to predict the prognosis of advanced gastriccarcinoma.