Mechanism Study In The Effect Of Yi Qi Qing RE Prescription On Theaffection Of GES-1 Infected With Helicobacter Pylori With Vaca And Caga

Helicobacter pylorus is a primary etiological agent leading to chronic gastritisand peptic ulcers. The organism is associated with gastric cancer in experimentalanimal models and in epidemiological studies. Although the World HealthOrganization concluded that H. pylori is a definite groupⅠcarcinogen.1. Therapeutic action of medicine on the athymic mouse infected with Helicobacterpylori with vacA and CagA1.1Influence of medicine on the body weight of the athymic mouse infected withHelicobacter pylori with vacA and CagAComplex prescription group and Yiqi group can both lessen significantly thedegression of the body weight of the athymic mouse infected with Helicobacterpylori with vacA and CagA. And this hints that Complex prescription group andYiqi group have the effect in inhibiting the degression of the body weight of theathymic mouse infected with Helicobacter pylori with vacA and CagA.1.2 Influence of medicine on the stomach coefficient of the athymic mouse infectedwith Helicobacter pylori with vacA and CagAYiqi group can significantly degrade the stomach coefficient of the athymicmouse infected with Helicobacter pylori with vacA and CagA compared with themodel group. This hint that Yiqi group has the effect in lightening the inflammationexudation in the stomach of the athymic mouse infected with Helicobacter pyloriwith vacA and CagA1.3Influence of medicine on the infection rate and the affection of gastric mucosa ofthe athymic mouse infected with Helicobacter pylori with vacA and CagAWe can find that the infection rate of Helicobacter pylori with vacA and CagAraised and gastric mucosa of the athymic mouse infected with Helicobacter pyloriwith vacA and CagA show up the severe affection. The infection rate ofHelicobacter pylori with vacA and CagA decreased and the affection of gastricmucosa of the athymic mouse also relieve compare with the model group, Yiqi andQingre prescription. Yiqi and Qingre prescription can also lighten the infection rateof Helicobacter pylori with vacA and CagA compared with the model group. 2. The intervention effect of medicine on pathological changes of the normal gastricmucosa cells affected with Helicobacter pylori with vacA and CagA2.1 The intervention effect of medicine on apoptosis of the normal gastric mucosacells affected with Helicobacter pylori with vacA and CagA——flow cytometryThe apoptosis rate of the model group intreased significantly compared withthe normal group and the apoptosis rate of the model group decreaded significantlycompared with the model group. We can conclued that Yiqiqingre prescrription canlower the upgraded apoptosis rate induced by Helicobacter pylori with vacA andCagA.2.2The intervention effect of medicine on apoptosis of the normal gastric mucosacells affected with Helicobacter pylori with vacA and CagA——hoechst 33258The model group appeard the karyopycnosis and the Yiqiqingre prescriptioncan improve the apoptosis phenomenon.2.3The intervention effect of medicine on the cyclomorphosis of the normal gastricmucosa cells affected with Helicobacter pylori with vacA and CagA——flowcytometryThe cells in the G0-G1 stage decreased a little and the cells in the S stageincreased significantly compared with the normal group. And the Yiqiqingreprescrription can lower the cells in the S stage.2.4 The intervention effect of medicine on the expression of cox-2 proteinum of thenormal gastric mucosa cells affected with Helicobacter pylori with vacA and CagAThe expression of cox-2 proteinum of the normal gastric mucosa cells affectedwith Helicobacter pylori with vacA and CagA increased significantly. When weadded the serum with Yiqiqingre prescription and find that the expression of cox-2proteinum decreased.2.5The intervention effect of medicine on the expression of p53 proteinum of thenormal gastric mucosa cells affected with Helicobacter pylori with vacA and CagAWhen GES-1 cells were cocultured with Helicobacter pylori with vacA andCagA, the expression of p53 proteinum increased significantly. The Yiqiqingreprescription can significantly lower the expression of p53 proteinum compared withthe normal group. The Yiqi and Qingre prescription can also a little lower theexpression of p53 proteinum.2.6The intervention effect of medicine on the expression of bcl-2 proteinum of thenormal gastric mucosa cells affected with Helicobacter pylori with vacA and CagA When GES-1 cells were coculmred with Helicobacter pylori with vacA andCagA, the expression of bcl-2 proteinum increased significantly. The Yiqiqingreprescription can lower the expression of bcl-2 proteinum of the normal gastricmucosa cells induced by Helicobacter pylori with vacA and CagA. The Yiqi andQingre prescription can also a little lower the expression of bcl-2 proteinum.2.7The intervention effect of medicine on the expression of ki67 proteinum of thenormal gastric mucosa cells affected with Helicobacter pylori with vacA and CagAWhen GES-1 cells were cocultured with Helicobacter pylori with vacA andCagA, the expression of ki67 proteinum increased significantly. The Yiqiqingreprescription can lower the expression of ki67 proteinum of the normal gastricmucosa cells induced by Helicobacter pylori with vacA and CagA. The Yiqi andQingre prescription can also a little lower the expression of ki67 proteinum and theYiqi prescription is power than the Qingre prescription.2.8The intervention effect of medicine on the expression of PCNA proteinum of thenormal gastric mucosa cells affected with Helicobacter pylori with vacA and CagAWhen GES-1 cells were cocultured with Helicobacter pylori with vacA andCagA, the expression of PCNA proteinum increased significantly. The Yiqiqingreprescription can lower the expression of PCNA proteinum of the normal gastricmucosa cells induced by Helicobacter pylori with vacA and CagA. The Yiqi andQingre prescription can also a little lower the expression of PCNA proteinum andthe Yiqi prescription is power than the Qingre prescription.