Experimental Study Of Gene Polymorphism And SNP Motion Insomnia Tyrosine Hydroxylase

Motility is a special type of insomnia, is also one of the symptomsof exercise-induced fatigue. Moderate exercise can help sleep, but beyond a certainlimit is likely to lead to insomnia. Once the researchers confirmed that the motility ofinsomnia has certain transmissibility. Catecholamine is important hormones andneurotransmitters in the human body, By tyrosine synthesis, there are four kinds ofenzymes involved in synthesis process, tyrosine hydroxylase was the first enzyme thatcatalyzed tyrosine produce dopamine, On regulating central nervous system and thesympathetic nervous system plays an important role, Due to TH play an important rolein regulating the central nervous, this article advance from TH gene level to discussmotility insomnia.Purpose: Through the questionnaire survey, sequencing the PCR technology, Weanalyzed Tyrosine hydroxylase gene SNP genotyping In the group of insomnia andinsomnia,and Investigated tyrosine hydroxylase gene polymorphism and motilityinsomnia occurs if there is a certain correlation.Methods: Using questionnaire survey to survey the basketball class, martial arts,weight lifting, aerobics class, track and field, wushu team, basketball team430athletes.Insomnia with motility evaluation criteria to the analysis judgment, The subjects weredivided into insomnia and insomnia group, Application of PcR-RFLP analysis method,Insomnia group of16people is obtained by sequencing of tyrosine hydroxylase genesequence and the insomnia group of18people of tyrosine hydroxylase gene sequences.By comparing the insomnia and insomnia group tyrosine hydroxylase gene SNPpolymorphism, Judge whether the tyrosine hydroxylase gene has certain connectionwith the motility of insomnia.Result:1, determination of fatigue of self-conscious symptom,67%of the scorein the fourth grade (19-24points).2, Weightlifter100%body fatigue after exercisetraining, internal fatigue in20%,9%in mind fatigue; Basketball, martial arts,gymnastics, track and field, and there was the probability of fatigue, respectively,60%,52%,50%,36%.3, from the analysis G-494A,C-388T,A-641G of experimental data oftyrosine hydroxylase gene loci of parting in motility insomnia and insomnia group,And G-94T, A-581G, T-741C, G-801C, G-814A, C-824T, C-833T no statisticaldifference.Conclusion:1, after67%of the athletes in sports training will appear in thehigher degree of fatigue.2, weight lifting athletes in body fatigue ratio is larger, appearsmaller ratio of fatigue and fatigue of the viscera. Basketball, martial arts, gymnastics,track and field athletes appear larger ratio of fatigue and fatigue of the viscera.3, tyrosine hydroxylase gene loci G-494A,C-388T,A-641G polymorphism associatedwith motility of insomnia has certain signs, We can’t prove that tyrosine hydroxylasegene loci G-494A,C-388T,A-641G is associated with motility insomnia is exact, Andtyrosine hydroxylase gene G-94T, A-581G, T-741C, G-801C, G-814A, C-824T,C-833T did not prove their relevance.