Semen Extraction Separation And Purification Of Total Saponins

Wild jujube (Ziziphi Spinosae Semen) seed is the dried ripe seed Rhamnaceae Ziziphus jujube, fill with liver, heart, perspiration, Sheng Jin effect for insomnia, dreaminess, palpitation, hyperhidrosis, Tianjin injury thirsty syndrome. Compendium of Materia Medica as its top grade. Suanzaoren high medicinal value, its pharmacological effects have been studied. This paper mainly focuses on process optimization direction made the following research, for further development and utilization of Chinese jujube kernel provides valuable reference.Firstly, establishing the method of determining quantities of total saponins and saponins A of Semen Ziziphi Spinosae in Ampelopsis Grossedentata.Take aponins A of Semen Ziziphi Spinosae as control article, adopt ultraviolet spectrophotometry to wavelength scan by total saponins control article in the200-700nm and recognize472nm is a maximum absorption W/L. Take saponins A of Semen Ziziphi Spinosae as control article to determine contents of saponins A of Semen Ziziphi flow number is1.OmL·minute; sample size is20μL; column temperature is25℃.Secondly, optimating the extraction techniques of total saponins. By the crosscut experimentation, in the reference indicatrix of total saponins extraction percentage、saponins A of Semen Ziziphi Spinosae extraction percentage、total saponins contents and saponins A of Semen Ziziphi Spinosaecontents to pick out the best abstraction artwork:material ratio is1:10; alcohol density is70%; abstraction temperature is70℃; abstraction times is two. The study provid capital theory parameter for Chinese materia medica gallipots dynamic state countercrrent distribution.Thirdly, to study the technological parameters of the purification process of saponins of Semen Ziziphi Spinosae with maeroporous adsorption resin. The adsorptive characteristics and elutive parameters of the process of total saponins semen ziziphi spinosaecontents were studied by taking the elutive and purified ratio of saponins as markers.saponins were collected in the70%ethanol. the elutive ratio of saponins was91.77%and the purity reached298.75%. The process of applying maeroporous adsorption resin to adsorb and purify total saponins of Semen Ziziphi Spinosae is feasible.Finally, preliminary application of defoaming agent in Jujuboside concentration process. With defoaming rate and surface tension as the indexes of six kinds of the defoaming performance, and study of concentration and temperature, defoaming agent concentration and defoamer usage for the influence of antifoaming effect. Selection of food defoaming Wang defoaming agent, concentration and temperature of70℃, defoaming agent concentration of0.08g/mL, dosage of20mL, concentration and time can be reduced to the original50%, surface tension can be reduced by0.01683N/m. Preliminary study on the defoaming agent can be applied in the Jujuboside concentration process.