| Mylabris is a Chinese traditional medicine. It is specified in Chinese Pharmacopoeia（2005 version） that Mylabris is the dried body of Mylabris phalerata Pallas. And Mylabris cichorii Linnaeus. In the present dissertation, methods of quality control of Mylabris and complex prescription of Mylabris, and the pharmacokinetics of Mylabris in different decoction were studied.A GC method was developed for the determination of cantharidin in Mylabris. The vanillin was used as the internal standard. The cantharidin and interal standard,were separated on SE-54 glass capillary column（30 m×0.25mm i.d） with hydrofen flame ionization detector. The temperature of injector was at 200℃, detector at 230℃, column at 180℃.The linear range was 12.5～600μg·mL-1. The standard curves was Y =6.330 X+3.240×10-2 and the correlation coefficient was 0.999 4. The recoveries were 98.5 % with a coefficient of variation of 4.0%.Fatty acid in Mylabris was determined by GC method. The extracted fatty acids were esterified and analyzed by a 30 m×0.25mm i.d. PEG-20 M glass capillary column and FID. The separation was established with programmed temperature, and the temperature of injector was at 200℃, detector at 230℃. The linear range of the tetradecanoic acid methyl ester was 2.325×10-3～0.465 0μg·mL-1, and the palmitic acid methyl ester was 1.0～15.0 mg·mL-1.Atomic absorption spectrometry methods were developed for trace metal elements（Fe, Mn, Cu, Zn, As and Hg） in Mylabris. The standard curves were linear over working ranges and gave average correlation coefficients between 0.997 5-0.999 8. The precisions were between 0.9 %-6.9 %, and the recoveries were between 80 %-120 %. The assay methods are simple, rapid and with good reproducibility and provide a quantitative basis for the quality assessment for Mylabris.The optimal esterify process of fatty acids was studied by orthogonal design with a area of fatty-acid methyl ester as indices. Three factors were studied in this experiment, including the temperature, reaction. time, the ratio of BF3-ethylether and methanol. The result showed that the optimal extracting condition was 60℃,20 min and the BF3-ethylether to methanol（2:1）.Several groups of animals were given the drugs with different intervals of time to work out the pharmacokinetics parameter of Mylabris. The half lethal doses of Mylabris was 344 mg·kg-1. The apparent removal rates were 0.1054 h-1. The apparent removal half-life periods were 6.58 h. The apparent distribution rate constants was 0.231 h-1. The apparent distribution half-life periods were 3.00 h. AUC was1493.4 mg·h·kg-1; CL was 0.132 mg·kg-1·h-1.A GC method was established to determine cantharidin in complex prescription of Mylabris. The linear range was 12.5～600.0μg·mL-1（r=0.999 4）, the recoveries were 100.2 % with a coefficient of variation of 1.6 %. An UV method was developed for the determination of glucos in complex prescription of Mylabris. The linear range was 0.080～0.80 mg·mL-1 and the correlation coefficient was 0.999 2, the recovery was 100.0 % with a coefficient of variation of 1.6%. |
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