Effect Of Aerobic Exercise And The Macrothele Raven Venom In H₂₂Tumor-bearing Mice

Objective:To observe the effect of the aerobic exercise combined the Macrothele raven venom on mice with H22liver tumor.Methods:50KM mice, H22mice liver tumor model constructed with subcutaneous transplantation. Modeling success mice were randomly divided into4groups:control group(C), venom low dose (VL), venom middle dose (VM) and venom high dose group (VH),10mice in each group with a tumor volume measurement every four days. After three days, the venom was injected in the venom groups through the tail vein once every two days, continuous administration for20days. The next day after stopping venom, overnight blood was taken and tissues of tumor were excised. The tumor inhibition ratios were calculated; Tumor cell morphology (HE stain), PTEN, AKT and mTOR protein, PTEN, AKT and mTOR mRNA expression were observed. Aerobic exercise (A) and the venom and aerobic exercise (VE) group were structured. After5weeks aerobic swimming exercise, the group of animals was treated as four venom groups.Results:Morphology of tumor cells in the C group appeared to be arranged densely. VL group cells arranged in irregular, small necrosis area in VM group and obvious necrosis in VH group, large necrotic area in VE group.PI3K, Bcl-2, AKT and mTOR expressions in VL, VM, VH and VE group were significantly lower. Bax and PTEN expression was significantly higher than C group.Conclusion:venom low dose, middle dose, high dose group and aerobic exercise combined venom group have an inhibition on mouse H22 hepatoma cell, and the latter is more effective. The mechanism may be related to the expression of the effective regulation of tumor cell factors, so as to achieve the inhibition of tumor proliferation.