| Objective:To determine the effects of aerobic exercise and the injection of Macrothele raveni toxin on tumor suppressor factors in mice with H22liver tumors.Methods:Forty healthy male Kunming mice were randomly divided into the model group, aerobic exercise group, heavy load aerobic exercise group, and the aerobic exercise combined with Macrothele raveni toxin group. Each group formed a subcutaneous xenograft model. The mice then underwent aerobic exercise with and without the administration of Macrothele raveni toxin.Measurements of tumor volume in each group were performed every four days, and a cell growth curve was constructed. The protein and mRNA expression of PI3K, Akt, mTOR, PTEN and IGF-1were determined.Results:Tumor growth rate was fastest in the model group and slowest in the aerobic exercise combined with Macrothele raveni toxin group. In the model group, tumor cells were arranged neatly and close together. In the aerobic exercise group, tumor cells were arranged loosely and irregularly and in the heavy load aerobic exercise group, tumor cells showed necrotic areas. In the aerobic exercise combined with Macrothele raveni toxin group, tumor cells showed large areas of necrosis. The expression of PTEN was significantly higher in the aerobic exercise group, the heavy load aerobic exercise group and the aerobic exercise combined with Macrothele raveni toxin group compared with the model no intervention group, while PI3K, Akt, mTOR and IGF-1were highest in the model group (P<0.01).Conclusions:The findings of the present study suggest that the administration of Macrothele raveni toxin in combination with aerobic exercise in mice injected with H22liver tumor cells had a beneficial effect by modulating the expression of PI3K/Akt/mTOR signaling pathway-related cytokines involved in the PI3K/Akt/mTOR signaling pathway, improved immunity and reduced injury due to H22liver tumors. |
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