| In this study, Acanthopanax fruit was as material. With the aim to provide theoretical basis and new methods for comprehensive utilization of new pigment.Extraction, depuration, stabilization, resistance to oxidation of Anthocyanins were investigated.Main conclusions were as follows:Compared with the solvent method and the ultrasonic extraction method. The optimal extraction environment of the solvent method was 45% ethanol, temperature was 70℃, iquid-solid ratio was 46:1, time was 4.5h, The content of anthocyanin was 828.26ug/g, by three effective extraction, the yield of anthocyanin reached 94.65%. And the optimal extraction environment of ultrasonic extraction method was 45% ethanol, temperature was 75℃, ultrasonic power was 400 W, liquid-solid ratio was 40:1, time was 65 min.The content of anthocyanin was 935.14ug/g, by three effective extraction, the yield of anthocyanin reached 95.83%. Because it had the advantage of short time and high extraction, the ultrasonic extraction method can be applied to extract Acanthopanax fruit anthocyanins.Four kinds of macroporous resin were compared, and D101 resin was determined to be appropriate for purifying the anthocyanins. The dynamnic adsorption test showed thatthe best adsorption concentration was 4mg/m L, the best sample flow rate was 1.5mL/min, the largest on the sample amount was 1100 mL. The dynamnic elution test showed that the best elution velocity was 1m L/min, the best elution solution was pH value of 2, 85% ethanol, the biggest usage of ethanol was 100 mL After the purification under the best condition, the color value was 10.81, which improved nearly five times before purification, the yield was 4.2%. The anthocyanins was dark red, uneasy to moisture absorption, easy to long-term preservation.The effects of different external condition on the stability of anthocyanins were discussed.The results showed anthocyanins were stable under acid, low temperature and dark conditions. Na+、K+、Ca2+、Mg2+ had no influence on its stability. Mn2+ had vigorous effect, Al3+(<0.05mol/L)' Zn2+(0.025mol/L) had promoting effect, further increases had inhibitory effect. Cu2+、Fe3+、Zn2+、Pb2+ had inhibitory effect, should avoid to contact. Oxidation resistance and the reduction of resistance are poor, Na2SO3 and H2O2 should be avoid to contact with anthocyanins. Glucose, sulfamate anf cane suger(>0.5%) had destructive effect.Acesulfame potassium had a smaller effect.Malic acid, citric acid, succinic acid and tararci acid(>0.25%) had better effect on color.Natrium benzoicum, sorbistat potassium, Vc and Sodium erythorbate had negative effects on the stability of the anthocyanins, should avoid to contact.The test of scavemging free radicals and reduction ability were discussed. The test of antioxidant capacity of Acanthopanax fruit anthocyanins showed anthocyanins can scavenge O-2·, when the concentration of anthocyanins was 3mg/mL, clearance rate reached 66.62%, IC50 value was 0.864mg/mL.Anthocyanins had significant scavenge on DPPH, when the concentration of anthocyanins was 300ug/mL, clearance rate reached 92.53%, IC50 value was 29.85ug/m L. Anthocyanins had negative scavenge on·OH, when the concentration of anthocyanins was 5mg/mL, clearance reached 30.44%. Anthocyanins had obvious reduction ability, presenting a tendency of increasing with the increase of the concentration and stabilizing after 700ug/mL. |
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