| Sargassum,belonging to sargassaceae, is an abundant resource of brown algae in our country, but has a low efficiency of comprehensive utilization. Studies have shown that sargassum contains abundant biological active substances like plant hormones, which have significant effects on plant growth, development andanti-adversity. At present, there are still few studies onsargassum plant hormones, and its utilization efficiency is usually very low. Therefore, in this research,plant hormones were extracted by using thebiological enzymolysis technology and the developed method of liquid chromatography-tandem mass spectrometry for simultaneous determination of four kinds of plant hormones in sargassum. By using the orthogonal design method for the best craft and usingthe content of plant hormones and polysaccharose as indicators, the quality of homemade sargassum source growth regulator was evaluated. The growth regulatorseventually passed through the bean sprouts, peppers and Chinese cabbage breeding,and then the tests of their growth were carried out. The main results of this study are as follows:1. The method for determination ofplant hormones from sargassum was optimized. Ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) can be used for simultaneous determination indoleacetic acid (IAA), indole butyric acid (IBA), abscisic acid (ABA) and zeatin (ZT) 4 kinds of plant hormones. The optimized methodof pretreatment and purification is:using 70% methanol as the extraction solvent withultrasonic treatment, and the concentrate was introduced to PCX+PAX solid-phase column,. The results showed that the recovery rate of fourkinds of plant hormones and relative standard deviationwere80-105%,1.06%-6.42%, respectively. With in 0.01-1 μg/mL, the correlation coefficient of various kinds of plant hormones was higher than 0.9990. The method of the detection limit is 0.025-0.2 μg/Kg.2. In this study, through the single factor design optimized Sargassum source plant growth regulator extraction process, ratio of material to liquid 1:3, the extraction temperature was 35℃, reaction time was 2h. The contents of IAA was 11.26 μg/Kg.The extraction conditions of Sargassum source plant growth regulator were optimized. By using the orthogonal design, the best conditions of enzyme solution were determined:dosages of cellulase and pectinase were 0.15 g/50g and 0.30 g/50 g, the enzymolysis temperature was 30℃, the reaction pH was 7, and the enzymolysis time was 3 h. By using the combination of extraction process and enzymatic hydrolysis process,the contents of the plant hormone:IAA 17.65μg/Kg, ABA 0.44 μg/Kg, IBA 0.95 μg/Kg, ZT 1.56 μg/Kg,and sargassum polysaccharose content is 2.42%.3. In this study,mung beans, chili seeds and Chinese cabbage seeds were used for test.By usingthe method of seed soaking,experimentsofseed germination and seedling growth were carried out to further study preliminary growth promoting effect. The results showed that in the seed germination experiment and seedling growth experiment, for bean sprouts, peppers and Chinese cabbage, germination potential, germination rate and germination index root length, plant height, fresh weight per plant, leaf area and chlorophy content were significantly influenced by 200 times and 400 times dilution of sargassum growth regulator.Choose 400 times dilution of Sargassum growth regulator for mung bean, pepper and Chinese cabbage seed germination and seedling growth. |
PDF Full Text Request