Extraction And Separation Of Ingredient Of Trichosanthes Kirilowii Maxim And Determination Of Antibacterial Activity Of Streptococcus Suis

Streptococcus suis is a common zoonotic infectious germs which can cause a variety ofdiseases of human and swine. At present, the serotypes of Streptococcus suis are numerous, sothe diseases that it causes mainly depends on the antibiotic treatment, and factors such as theunreasonable use of antibiotic in clinicl and the horizontal transmission between strains lead tothe appearance of resistance of Streptococcus suis and multiple drug resistance ia serious. It notonly brings difficulty to control and treat diseases, but also exacerbates the residue of antibioticin animal meat and milk products, threats the safety of animal food and what,s more, it,s easy tomake a resistance gene to be transferred to people by food and then make the treatment ofhuman diseases become a thorny problem. Therefore, the pharmacological experiment methodand the extraction and purification methods of Chinese medcinal herbs applied in the experimentto filter Chinese medcinal herbs which can restrain streptococcus suis, and combined withantibiotic. It can not only plays long-lasting and broad-spectrum antibacterial effect of Chinesemedcinal herbs, also highlight the characteristics of antibiotics whose pesticide effect are rapidand effect are obvious. The experiment was processed from the several aspects:1. The filter of inhibitor of Chinese medcinal herbs to Streptococcus suisWater decoction method and alcohol preparation formulation were used respectively topreparate twenty flavour extract of Chinese medcinal herbs, one multiple drug-resistant strainsStreptococcus suis which separated from clinical as tested bacteria, the screening resultsshowed that inhibitory bacterium additive effect was existed in the alcohol extract ofTrichosanthes kirilowii maxim, alcohol extract of smoked plum and water extract of smokedplum combined with ciprofloxacin to multiple drug-resistant Streptococcus suis by thecheckerboard method.2. Active ingredient tracking of extracts of Trichosanthes kirilowii maxim to the StreptococcussuisExtract as the order of petroleum ether, chloroform, ethyl acetate, n-butanol according topolarity from small to large and extracts were obtained. To determine minimum bacteriostaticconcentration (MIC) of ciprofloxacin and extract of Trichosanthes kirilowii maxim toStreptococcus suis strain of Pf–cc by Chinese and western medicine combined drug susceptibility tes. Screening for the extract which can improve the sensitivity of bactera. Theresults showed that N-butanol extract combined with ciprofloxacin showed added bacteriostaticaction.Isolating and puring of Chinese medcinal herbs active ingredients by columnchromatography, thin-layer chromatography, silica gel column chromatography and gel columnchromatography (Sephadex LH-20) and other means. Identification the structures of compoundsaccording to the physical and chemical properties and spectral data.3. Research of effective components such as Saponin compounds of Trichosanthes kirilowiimaximExtract of Trichosanthes kirilowii maxim was obtained by condensing reflux extraction.Water solution of concrete was added to polyamid resin column and macroreticular resin column,then eluent of ethyl alcohol of different concentration were obtained and contents weredetermined. Ultimately flavonoids and saponins of Trichosanthes kirilowii maxim wereobtained.To determine minimum bacteriostatic concentration (MIC) of ciprofloxacin and extract ofTrichosanthes kirilowii maxim to Streptococcus suis strain of Pf–cc by Chinese and westernmedicine combined drug susceptibility tes. The results showed that polyamide eluent of10%,70%ethanol and macroporous resin of10%,70%,95%ethanol elution all have bacteriostaticactivity to Streptococcus suis. Polyamide10%ethanol elution combined with ciprofloxacinshowed additive bacteriostatic action.