Studies On Two-Step Cultivation And The Accumulation Of Active Component Of Dendrobium Candidum

Dendrobium candidum is also called the Den.Sharifa Fatimah, belonging to the orchid Dendrobium, Supervenience perennial herbaceous plants, which fresh or dried stem can be used as herbs, the main efficacy composition of polysaccharides and alkaloids.The seed germination rate is quite low through the single cultivation, symbiosis with fungi can sprout. In addition Dendrobium candidum growth in damp environment,natural and artificial cultivation is very slow, harvest yield is low and in short low. In recent years, people has carried out a large number of studies on the rapid propagation, successful differentiation induction and rapid propagation. However, test tube seedling propagation coefficient is high and fast growth, but often active component is low, and have higher plant growth regulators in plant residues, affect drug safety. Tissue culturecan accelerate the breeding and promotion of test-tube seedling growth, increase biological yield quick;the soil cultivation promote the synthesis of active component. This experiment combined with high propagation coefficient, fast growth, soil cultivation conducive to effective composition and drug safety advantages, set up a tin of caulis dendrobii staged training and cultivation mode, set up a tin of caulis dendrobii staged training and cultivation mode1. Vaccination somaclone in the form of micro cutting, its effect is better than that traditional tissue culture.Cytokiues and cytokinin under the combination effect is good for1/2MS+0.5mg/L NAA+1.0mg/L6-BA, vigorous growth, green color and luster. Cultivation of40d in culture conditions, long out of the lateral bud and the average biomass growth of strain4.52g/S. The process of seedling rooting of Dendrobium candidum plantlets, the most favorable combination is1/2MS+0.5mg/LNAA+1.5mg/L6-BA, vigorous growth, green color and luster. Shorter than traditional organizational culture time and achieved the purpose of earth micropropagation as production test tube seedlings.2. Sheet metal in the process of tissue culture of dendrobium stem appropriate PH value of5.6, the most suitable temperature control in the25±1℃, this temperature somaclone growth is best,but the test-tube seedlings cultivation temperature in15to28℃and conducive to the active component accumulation; the growth of Dendrobium candidum is strict with light, seed lighting control in the1400lx, illumination time12h/d, the light of tissue culture seedlings grow well, Dendrobium candidum plantlets rooting light control in about3000Lx, illumination time is12h/d, The bud seedling under the light, color is dark green, strong root and tidy.3. Laboratory imitation of suitable growth temperature, light intensity and illumination time. Night temperature in15-18℃, during the day temperature25-28℃suitable for the active component composition of dendrobium candidum. The light shines in the7500Lx, illumination time12h/d under the conditions of Dendrobium candidum growth strong, deep green seedlings. Spraying nutrient solution contains a lot of elements, once a month with1/3times of the standard solution, best before spraying nutrient solution PH value of5.6.4. Organization culture can speed up the breeding and promotion of test-tube seedlings growth, rapidly increase the biomass production; the soil cultivation promote the synthesis of active component. Combining tissue culture and humus soil cultivation technique, set up iron caulis dendrobii phase type culture and cultivation mode. By further optimization of caulis dendrobii staged training and cultivation mode,will be conducive to improve the Dendrobium candidum medicinal material production.