Effect Of Fluoride On Y Chromosome Microdeletion Gene And Its Meachanism Study In Male Mice

[Objective] Spermatogenesis is in the convoluted tuble of the male animal testis, the germ cells from spermatogonia, developed to the primary spermatocyte and then developed to the secondary spermatocyte, to sperm cells, finally developed into sperm. The process of spermatogenesis is easy to be damaged by external poison. This study successfully establishde the animal model, because we find when we exposed to NaF the male reproductive system is damaged and leading to the spermatogenesis failing.This study is to detect whether fluoride would affect spermatogenesis associated with the Y chromosome microdeletions gene, and then to reveal the underling mechanisme of fluoride toxicity in sperm chemotaxis if it does.[Method]40healthy Kunming male mice(8weeks old) were randomly divided into4groups and exposed to25,50,100mg NaF/L in their drinking water for11weeks. During esposuring periods,Weight is regularly recorded.At the end of the esposure periods, F concentration in bone testis of organs coefficient, epididymis of organs coefficient, Sperm count, Sperm malformation ratio, Sperm head malformation ratio, serum testosterone and tissue testosterone was measured; To observe the testis tissue slice under the light microscope. The Y chromosome microdeletions gene and HSF2were determide to elcidate the possible molecular mechanisms.[Results]1.After11weeks expousre, there were no significant difference in body weight and major organs coefficient were observed between NaF treatment groups and control groups. Fluorine contents in bone25mg/L,50mg/L and100mg/L NaF treatment group showed significant difference compared with control group. Sperm count is no significant difference. Sperm malformation ratio100mg/L NaF treatment group showed significant difference compared with control group. Serum testosterone100mg NaF treatment group showed significant difference compared with control group. Besides, HE staining photos showed that high does fluoride would damage the structure of testis.2.According to the results of QRT-PCR, level of Sly mRNA were decreased in the higher treatment groups.Ssty2mRNA were decreased in the middle and higher treatment groups. Whereas, The other mRNA of trget genes detectde no statistical difference. 3. According to the results of QRT-PCR, level of HSF2mRNA were decreased in the higher treatment groups. The result of ELISA test showed that HSF2were decreased in the higher treatment groups.[Conclusion]Taken together, our study showed that fluoride would harm to the male reproductive system and lead to spermatogenesis failing by administrating with50and100mg NaF/L in drinking water for11weeks..The toxicity mechanism may include the decent of Ssty2mRNA,Sly mRNA and HSF2mRNA expression and HSF2protein expression.