Study On Tissue Culture Rapid Propagation Of Sinopodophyllum Hexandrum In Taibai Mountain

Sinopodophyllum hexandrum(Royle)Ying，Perennial herb, is one of "the best sevendrugs in Mountain Taibai ".and it ’s the wild rare and endangered medicinal plants inMountain Taibai. In this study, Sinopodophyllum hexandrum(Royle)Ying ’ leaves, stems,roots and embryo are used as explants to study the different mediums, hormone levels,lighting conditions, and browning control of effects on callus induction,and the effects onadventitious buds and proliferation of different hormones and lights on adventitious buds andproliferation, temperature and other conditions, the impact on rooting by differenthormone.Then,this experimental study explored a complete plant regeneration system by thisway.This study conducted a preliminary exploration on the process of somatic embryogenesisused the embryo as explants and induced somatic embryos successfully. The main results areas follows:1The embryo was inoculated on the1/2MS medium,B5medium,WPM medium andMS medium which added1.0mg·L-12,4-D. The callus in the MS medium has highestinduction rate, the B5medium second and the1/2MS medium has worst induction rate.2The aseptic seedling was induced by using mature seeds as explants. The best mediumwas the MS medium which added2.5mg·L-16-BA and0.6mg·L-12,4-D.3This study used Sinopodophyllum hexandrum’s mature embryo,roots,stems and leavesof aseptic seedling as explants to induce callus on the MS medium. The tissue culture systemof Sinopodophyllum hexandrum increased callus induction rate significantly used matureseeds as explants,and the rate was higher than the callus of root, stem and leaves asexplants. Induction of medium supplemented with TDZ was much higher than the Mediumadding2,4-D.The highest induction was MS medium added0.5mg·L-1TDZ+500mg·L-1CH,the rate up to97.8%. But, higher concentrations of2,4-D, TDZ all have a disincentive to thecallus induction.4Somatic embryogenesis subculture can induce somatic embryos using TDZ. But thegrowth arrest after globular and heart-shaped embryo appeared, and not found torpedo-shaped or cotyledon-shaped embryos. The browning was extremely severe insubculture process. Browning problem can be resolved using GSH160mg L-1.5The MS medium with6-BA2.0mg L-1and NAA0.5mg L-1is most suitable toinduce bud differentiation and proliferation.The bud differentiation rate was60.4%and theproliferation of shoots was5.2, significantly higher than other treatments.The process ofadventitious buds proliferation that during the first7days of dark culture, then light trainingwas the best treatment.6Used MS as basic medium, which added0.5mg·L-1IAA and0.5mg·L-1NAA to inducedifferentiation roots. The results showed that the medium which added of0.5mg L-1IAAhas the highest rooting rate of22.4%.