The Effects Of Stellera On The Rhizospher Microbial Community And The Protein Expression Profile Of Chamaejasme Under Drought Stress

Stellera chamaejasme belongs to Thymelaeaceae Stellera genus, is one of the main toxic grass spread on China’s western grassland the grasslands in Western china. Although the whole plant is toxic, in the period of spring grass regreening sometimes may still resulting the cattle and sheep poisoning and even death by eating in accident. Furthermore, the spread of Stellera chamaejasme result inthe degradation of other high quality forage grass because of its strongly ecological competitiveness, and it brings greatly economic losses to the general herdsmen. This paper will introduce two main aspects:the first part is to study the influence over the growth effects of Stellera chamaejasme on rhizosphere soil microbial community structure by using the soil macro gene sequencing method; The second part to study the protein expression of Stellera chamaejasme under drought stress using two-dimensional electrophoresis, and measurement of the changes of photosynthetic indexes of Stellera chamaejasmeblade stress process through the photosynthetic apparatus. And this work mainly achieved the following results:1、compare the stellera around rhizophere and rhizosphere soil, microorganism macro gene sequcing shown that there’s non-obvious influence on bacterial communities but there has obvious influence on composition of it from the growth of Stellera chamaejasme. At the gate level contrast stellera rhizosphere and non rhizosphere fungal community structure, Ascomycetes accounted for more than 60% in non rhizosphere soil, but in rhizosphere soil, basidiomycotina fungi accounted for the most(more than 60%). But from a comparison of the genus level, coral fungi of the genus basidiomycetes in Stellera chamaejasme in rhizosphere soil accounted for more than 30%, fungi of the genus Archaeorhizomyces accounted for nearly 10%, but these two kinds of fungi concentration in the non rhizosphere soil was almost negligible. Therefore, the growth of Stellera chamaejasme is closely related to this two types of bacteria.2、It was found that the maximum net photosynthetic rate, stomatal conductance, transpiration rate decreased under prolonged drought stress, while the intercellular CO2 concentration and NPQ under drought stress time prolonged rise, while the chlorophyll fluorescence index of Fv/Fm, Fv’/Fm’, ETR decreases. Because it has the same variation tendency with other general plant photosynthesis under drought stress, weuse the photosynthetic measurement to study the nature of stellera under drought stress is doable.3. compare the Stellera chamaejasme protein expression profile before and afterd rought threats. Through the HPLC-MS method identified 19 kinds of expressed proteins. The ribulose 1,5-bishosphate carboxylase subunit and Chlorophyll a-b binding protein expression quantity clear cut after drought stress,they are all key protein during photosynthesis. It’s comford to the photosynthetic rate dropped significantly. Fructokinase is the key enzyme in the glycolysis and ATP synthase subunit is the key enzyme in ATP synthesis. The expression quantity of these twoenzyme clear cut after drought stress. Showed the drought stress impact of the basic metabolism for Stellera chamaejasme. CIP proteolytic enzymes and proteasome have intimate connection with damage protein removal. The expression of this two enzymes quantity increases obviously. This may be a protective mechanism of stellera. Formate dehydrogenase specifically expressed after drought stress, The enzyme can affect mitochondria and chloroplasts protein synthesis,Nucleotide synthesis and Metabolism of vitamins. This enzymes play an important role in the process of plant response to stress. ThePP2C like protein kinase specifically expressed after drought stress too. This protein can be mediated by reversible protein phosphorylation, it play an important role in the cell signal transduction and stress response. Most of the protein kinase gene can be activated when drought, low temperature, high salt, uv lamp intimidation, these kinds of enzyme can improve the ability of plants to cope with stress.