Effects Of Immune Function And Expression Of β-defensin-1mRNA In Immune Organs On Probiotics-Fed Chicks

One day old chicks fed with probiotics were used in this study. The technologies of tissue paraffin section and histochemical staining and real-time fluorescence quantitative PCR were used to respectively research the following details in immune organs—bursa of Fabricius、spleens、thymus of chicks fed with different kinds of probiotics: the plasma cell number and ratio of nucleus, the number of TANAE+ cells and the dynamic changes of Gallinacin-1(Gal-1) m RNA expression. Our objective is to comprehensive understand the influence of different types of probiotics on chicks immune organs immune function and the expression of Gal-1m RNA, and provide scientific experimental basis for the research of probiotics. The results were as follows:(1) Chicks fed with Bifidobacterium, the number of TANAE+ cells in thymus、spleen、bursa were higher than that of the control chicks(P<0.05) at 7~13 days; the number of plasma cells in spleen、bursa and thymus were higher than that of the control chicks(P<0.05) at 10~13days, 7~10days, respectively; while the Gal-1m RNA expression in above immune organs is significantly higher than that of the control chicks(P<0.05 or P<0.01) at 10~13 days.(2) Chicks fed with Lactobacillus, the number of TANAE+ cells、 plasma cells and the Gal-1m RNA expression in spleen and bursa were higher than that of the control chicks(P<0.05 or P<0.01) at 7~13 days, while the number of plasma cells and TANAE+ cells was higher than that of the control chicks(P<0.05) at 7~10 days; while the Gal-1m RNA expression in above immune organs is significantly higher than that of the control chicks(P<0.05 or P<0.01) at 7days.(3) Chicks fed with Bifidobacterium and Lactobacillus in combination, the number of TANAE+ cells in thymus、spleen、bursa were higher than that of the control chicks(P<0.05) at 7~10 days、13days and 10~13days; the number of plasma cell in above immune organs were higher than that of the control chicks(P<0.05) at 7 days.While the Gal-1m RNA expression in above immune organs is significantly higher than that of the control chicks(P<0.05 or P<0.01) at 10~13 days.The effect of Bifidobacterium to the number of plasma cells and TANAE+ cells, and the Gal-1 m RNA expression is better than others.