Tissue Culture And Rapid Propagation Technology Of Rubus Crataegifolius Bunge And Lycium Ruthenicum Murr.

In this study, the Rubus crataegifolius Bunge and Lycium ruthenicum Murr.were mainly used as experimental materials.With their tender stems, through induction, multiplication culture, induced to take root germination, seedling transplanting, and soil culture researching, tissue culture rapid propagation system was established. 1. Tissue culture and rapid propagation technology system of R.crataegifolius BungeNew germination of stem segments were used as explants in June, and the best method of disinfection explant was, immersed disinfection for 30 s with 75% alcohol and 0.1% HgCl2 for 10 min.The MS medium, with 0.5mg/L 6-BA and 0.2mg/L NAA, was the properest for the primary cultivation.The best proliferation medium was MS + 6-BA 1.0mg/L + NAA 0.2 mg/L. At this time, the proliferation rate was the highest and reached 33.14. The most suitable rooting medium was improved 1/2MS+ NAA0.10mg/L.(Rooting rate was 100%, and the average number of roots up to 11.21, with an average root length up to 4.86 cm.) When getting on rooting ex vitro, the most appropriate concentration of NAA was 200mg/L. The average rooting rate was 82.5%,(with the average number of roots up to 11.9,and the average root length was 3.2cm). The survey result of the seedings growth and development situation showed that mid-June was the best time to transplant the seenlidgs into soil. 2. Tissue culture and rapid propagation technology of L.ruthenicum Murr.New germination stems of L.ruthenicum Murr.were used as explants.They were immersed disinfection for 30 s with 75% alcohol and 0.1% HgCl2 for 5 min.Then inoculated on the MS culture medium,with 6-BA 0.5mg/L and NAA 0.2mg/L. The most appropriate medium was MS + 6-BA 0.2mg/L + NAA 0.01 mg/L.The most suitable rooting medium was improved 1/2MS + IBA0.1mg/L+ NAA0.2mg/L. Rooting rate of it could up to 72.2% on average, and the roots and seedlings grown stoutly.