Study On The Technique System Of Tissue Culture And Rapid Propagation Of Lycium Ruthenicum Murr

Because of its significant medicinal value and unique botanical characteristics, the Lycium ruthenicum Murr. was began to be known and utilizde. At present, Lycium ruthenicum Murr. was almost breeding with seeds, because its fruit were smaller, when picked which needed a lot of labor, it brought in no convenience and high composition in the same time. Therefore, the group studied the technique system of tissue culture and rapid propagation of Lycium ruthenicum Murr. which were rapid propagation with the method of tissue culture, provided a theoretical basis for the breeding of Lycium ruthenicum Murr.. The results showed that:1. The suitable medium for axillary buds of original culture was MS+ZT0.2 mg·L-1+IBA0.01 mg·L-1, on this medium, the speed of germination of axillary buds was quickly, the axillary buds germination rate reached 88.73%, the buds are green, and the vitrification was rarely.2. The effect of ZT on the proliferation of tissue cultured plantlets is better than the 6-BA, the suitable medium for proliferation is MS+ZT0.15 mg·L-1+IBA0.01 mg·L-1, on this medium, the growth rate of plantlets was quickly, the proliferation rate was 5.83 times, tissue culture seedlings basically no vitrification, and seedling grew well.3. On the progress of the proliferation culture of Lycium ruthenicum Murr., increase the light intensity to 4000 lux, light time to 16 h·d-1, concentration of agar to 6.0 g·L-1, which could reduce the vitrification of tissue culture seedlings effectively; adjust the concentration of ZT in circle, the week of circles were 4 times proliferation culture, the first proliferation culture concentration of ZT was 0.15 mg·L-1, when increased the proliferation culture times, reduced 0.02 mg·L-1 every time, when concentration of ZT effected the proliferation times, increased 0.02 mg·L-1 every time again, when the fifth proliferation culture, adjustd the 0.15 mg·L-1. Adjustd the concentration of ZT by circles, which could reduce the vitrification of tissue culture seedlings effectively.4. The suitable medium for rooting of tissue cultured plantlets was MS+IBA1.0 mg·L-1, on this medium, the growth rate of plantlets was great, the rooting rate could reach 100%, meanwhile, the roots were strong and no callus.5.Humus: Pearlite=1:1 was relatively suitable transplanting substrate for Lycium ruthenicum Murr. which had the best effect, on this medium, the growth was better than other combinations of matrix, and the survival rate was 92.37%.