Study On Genetic Giversity Of110Maize Inbred Lines

Researching on genetic diversity of maize inbred lines and classifying genetic relationshipbetween different groups are significant to the germplasm innovation and breeding of maize.This study is aimed at clarifying the genetic relationships among110maize inbred lines, so asto make a better use of and improve maize inbred lines, providing the basis for breeding infuture. Genetic diversity of inbred lines was studied in the method of morphological and SSRmarker, and the results are as followed:(1) Relational degree between yield, growth period traits and other traits of110maizeinbred lines were analyzed respectively using Grey Relational Analysis. It is concluded thatthe closest three correlations with single spike grain weight traits are grain number, hundredgrain weight and spike thick; And the three lines of grain number correlation is panicle length,ear thickness and plant height. The result shows that each character makes a clearlycontribution to the production, which provided a reference basis for high-yielding maizeinbred lines breeding direction and improved the efficiency of breeding.(2) The110normal maize inbred lines are diverse on the morphology of phenotypic traitsvariation, the coefficient variation of bald tip length is the maximum, the coefficient variationof leaf color is the minimum. ANOVA analysis for13characters including number of blades,tassel length, branches of tassel, ear thickness, rows, plant height, grain weight, ear height,row grains, axle weight, spike weight, bare top length and ear length. They have reachedsignificant level, indicating the existence of the real differences among the13traits. The110inbred lines are divided into11groups clustered by method of UPGMA using SPSS software.(3)28pairs of primers better in polymorphisms and stable in type were selected from45pairs of SSR primers.125alleles variables were detected from110inbred lines using thoseprimers. The allele number for the SSR loci ranged from2to10, with the mean allele being4.46. The PIC values for the SSR loci ranged from0.75to0.94, with the mean PIC being0.85and genetic similarity coefficient value is0.6527.110normal maize inbred lines were dividedinto six groups according to the result of cluster analysis.(4) It is concluded that the clustering results of morphology are consistent with that of SSRmarkers, indicating that the morphological markers can not be completely accurate to dividethe genetic relationship of inbred lines, but can reflect the morphological variationn amongdifferent materials to some extent. And morphological marker research provides a referencefor the research of SSR molecular markers.