Fertilization Effects On Physiological Property About Keteleeria Cyclolepis Seedling

Keteleeria cyclolepis, a member of Pinaceae, is evergreen species and endemic to China. It is an important species for both landscape and forest. In this research one year old seedlings from two provenances, Nanping, Fujian and Liupanshui, Guizhou was studied using orthogonal design (3x3). The objective was to explain how different fertilizer treatments affect both morphological and physiological growth of Keteleeria cyclolepis. The results were indicated as follows:1. In aspect of morphological charactoristics, different fertilization treatments showed a significant effect on the growth and physiological activity of Keteleeria cyclolep. Seedling height and ground diameter increased with a certain range of fertilizer increasing, however, after the range, too much fertilizer inhibited the growth of Keteleeria cyclolep seedlings. The treatment 5 (0.6 g N+1g P2O5+0.6 g K2O) was best to both height and ground diameter growth. In this treatment seedlings from Guizhou was in average height of 18.1 cm, increasing by 6.2 cm compared to pretreatment and 129% compared to contrast treatment; seedlings from Fujian was in average height of 27.1 cm, increasing by 5.6 cm compared to pretreatment and 166% compared to contrast treatment. In this treatment, compared to pretreatment and contrast, average ground diameter of Guizhou seedlings increased by 0.43 mm and 78%, respectively; average ground diameter of Fujian seedlings increased by 0.41 mm and 156%, respectively. Biomass of Keteleeria cyclolep seedlings from two provenances showed similar response to different fertilization. In the treatment 5 (0.6 g N+1g P2O5+0.6 g K2O), the biomass was the highest Compared to pretreatment, seedlings biomass of Guizhou and Fujian increased 187.5% and 147.5%, respectively. This response was similar to height and ground diameter. 2. In aspect of physiological characteristics, different fertilization treatments showed a significant effect on the physiological activity of Keteleeria cyclolep in a certain range. Treatment 9 (0.9 g N+1.5 g P2O5+0.4 g K2O) showed the most effect to leaf protain content of Keteleeria cyclolep seedlings. Compared to the contrast, the leaf protain content of Guizhou and Fujian seedlings in this treatment increased 90% and 95%, respectively. Treatment 7 (0.9 g N+0.5 g P2O5+0.6 g K2O) showed the most effect to the leaf soluble suger content; the leaf soluble suger content of Guizhou and Fujian seedlings in this treatment were 142% and 131% higher than the contrast. Treatment 8 (0.9 g N+1g P2O5+0.2 g K2O) affected the chlorophyll content; in this treatment the chlorophyll content of Guizhou and Fujian seedlings increased 85% and 109% compared with the contrast. Nitrate reductase content of Keteleeria cyclolep seedlings leaves was affected the most by treatment 5 Nitrate reductase content was 50% and 37% higher than the contrast in Guizhou and Fujian seedlings respectively.3. These results indicated that N-fertilizer showed higher effect on both morphological and physiological characteristics than P-fertilizer and K-fertilizer. The interaction of every two factors showed different effect to all the characteristics. Through the fitted quadratic polynomial multiplied regression equation, we found the optimum application amount of N, P and K.