Research On Multi-index Quality Evaluation Of Angelicae Sinensis Radix And Its Correlation With Soil, Altitude, Latitude, Longitude And Mineral Elements

In this paper, 151 batches of Angelicae sinensis Radix and its corresponding rhizosphere soil were collected from 15 county-level producing regions, Gansu Province and Yunnan Province. Based on the methods of ecology of traditional Chinese medicine resources and chemometric, the following 5 contents were systematically researched.A The correlation between the contents of chemical compositions and altitude, latitude,longitude and soil mineral elementsOur studies showed that the contents of ferulic acid, 3-butylphthalide and ethanol soluble extractive gradually increased with altitude in 2006 ~ 3080 m; the content of n-butylidenephthalide gradually increased with altitude in 2006 ~ 2548 m, but gradually decreased in 2548 ~ 3080 m; the content of Z-ligustilide gradually increased with altitude in2006 ~ 2732 m, but gradually decreased in 2732 ~ 3080 m; the content of volatile oil gradually increased with altitude in 2006 ~ 2641 m, gradually decreased in 2641 ~ 2824 m,then gradually increased in 2824 ~ 3080 m. Taken together, producing regions with higher altitude should be chosen for cultivating Angelica sinensis. The studies also found that Fe, Mn,Mg, K, As, Cr and Ni were the characteristic inorganic elements of Angelicae sinensis Radix.The elements of Zn and Mg, Cu and Ni, Fe and Mn, Ca and Na, Pb and Cu, As and Cr certainly showed effect of synergistic absorption, but K and Na, Pb and Cd certainly showed effect of antagonistic absorption. The decision factors of altitude on Mn, K and Cr, longitude on Ca, Na, Cd, As and Ni as well as latitude on Mg, Ca, Pb, Cd, As, Hg, Cr and Sb were positive values, which indicated that these corresponding geographic indicators were the main decision factors of the contents of these corresponding inorganic elements.B The correlation of the contents of mineral elements in rhizosphere soil of Angelica sinensis with altitude and soil typesOur reaches showed that Zn, Mn, Pb, Cu, Cr and Ni were the characteristic mineral elements in rhizosphere soil of Angelica sinensis. 15 County-level producing regions could be divided into 3 main producing areas:(1) Min County, Weiyuan County, Zhang County,Tanchang County and Wen County;(2) Lintan County, Kangle county, Hezheng County and Zhuoni;(3) 4 Counties, Yunnan Province. The contents of Mg and Cd in rhizosphere soilshowed significant positive and negative correlation with altitude, respectively. The contents of harmful element of Pb, Cd, As, Cu, Cr, Sb and Ni showed negative correlation with altitude.The distribution of mineral elements in rhizosphere soil were closely related to soil types, and the soil samples with the same or similar types tended to be classified in the same groups.Angelica sinensis had concentration for Na, belonged to the same level with K, Ca, Mg and Hg, relatively depleted for Zn, Cu, Pb and Cd, strongly depleted for Fe, Mn, Ni, As, Cr and Sb in rhizosphere soil. The inorganic elements of Angelicae sinensis Radix were closely related to the geological background of its rhizosphere soil. The contents of mineral elements in rhizosphere soil could affect the contents of corresponding inorganic elements in Angelicae sinensis Radix.C The application of NIRDRS in rapid contents determination of chemical compositions and identification of medicinal parts of Angelicae sinensis RadixWith the established quantitative model of NIRDRS-PLS(near-infrared diffuse reflectance spectroscopy-partial least square), the contents of Z-ligustilide, ferulic acid,n-butylidenephthalide, 3-butylphthalide and volatile oil could be rapidly determined. The results showed that the absorbance in characteristic absorption of NIRDRS fingerprints was in a decreasing order of body > tail > head > whole. The established model of PLS-DA(partial least square-discriminant analysis) could be used to accurately classify the different medicinal parts of Angelicae Sinensis Radix, which showed that the different medicinal parts of Angelicae Sinensis Radix have some difference in chemical components.D Research on NIRDRS fingerprintin and contents prediction of harmful metal elements in rhizosphere soil of Angelica sinensisOur reaches showed that the contents and types of chemical components in rhizosphere soil from Minxian County, Weiyuan County, Zhang County, and Tanchang County were more similar. The established quantitative model of NIRDRS-PLS could be used to predict the contents of harmful metal elements of Pb, Cd, Zn, Cu and Cr in rhizosphere soil of Angelica sinensis.E Research on the quality evaluation methods of Angelicae sinensis Radix based on its chemical compositionsWe established and compared the methods of analytic hierarchy process(AHP),entropy-weight, principal component analysis(PCA), and coefficient variation to determine the multi-index weights of the quality evaluation of Angelicae sinensis Radix, and found that the results based on AHP and entropy-weight were in line with the genuineness meaning and knowledge of traditional medicine experience. Then the models of TOPSIS(technique for order preference by similarity to ideal solution), PP(projection pursuit), and PCA were established to evaluate the quality of Angelicae sinensis Radix from different producing regions. The results showed that the Angelicae sinensis Radix from Min County, Weiyuan County, Zhang County, Tanchang County and Yunnan Province had better qualities, and more accord with the genuineness meaning and knowledge of clinical experience.