Genome Sequencing And Analysis Of DsRNA Virus Of Lentinula Edodes, And Diversity Analysis Of DsRNA Of Wild Strains

Complete browning of the substrate of Lentinula edodes’s fungus bag and fruit body deformity are bad phenomenons which occur in Fujian province. And this phenomenon result in serious economic losses. Scholars regard this phenomenon related to mycovirus.A cDNA library was constructed from a dsRNA purified from the fruiting body of L. edodes.17 primers were designed for a big band of dsRNA which were sequenced overall. The sequences were joint together by DNAman and build a whole sequence of 11282bp. And it contains two complete ORFs by analysis of ORF finder in NCBI. ORF1 encoded a protein composed of 1,975 amino acids, containing the conserved motif of a NUDIX domain (between 319aa and 451aa). ORF2 encoded a protein composed of 1426 amino acids, containing the conserved motif of RNA-dependent RNA polymerase (RdRp). The whole sequence is similar to L. edodes mycovirus HKB gene (AB429556.2) in 99%.Wild strains collected by our laboratory were detected with RT-PCR. We detect the distribution of dsRNA with the strains. Analysis of the virus of L. edodes was conducted to proving the virus band of wild strain according to electrophoresis picture of dsRNA and RT-PCR. The result shows that the existence rate of 11kb is 65.8% of 38 wild strains.According to electrophoresis picture of dsRNA and RT-PCR, we picked 14 strains for sequencing. Compare sequencing results with L20 corresponding fragments by multisequencing. Homology analysis of sequencing result could be classifid as three groups.