Comprehensive Prevention And Treatment Of Cow Mastitis In Large-Scale Farm

With the specification and management increasing of the dairy market in China, dairy-farm also towards large scale and standardization direction. Mastitis as the first of four major dairy disease, it is difficult to control, because of its complex causes. Mastitis could cause huge economic losses. We have inspected incidence of mastitis in one dairy farm in Tianjin. We have also separated the bacteria of clinical mastitis and tested the drug sensitivity of the bacteria. Combining the result with the feeding and managemen,we have formulated reasonable comprehensive prevention and treatment measures. The result proved that we had did a effective jobs.1. The survey of clinical mastitis This experiment have inspected 312 cows in dairy farm in Tianjin within seven months. A total of 60 heads infected clinical mastitis. The incidence of infection was 19.23%. We have found that the incidence had relationship with months. The incidence was different in different months, with the highest incidence in July and August. This result suggested that occurrence of mastitis is affected by temperature, humidity and other inviromental conditions. We should take effective measures to prevent as soon as possible in high incidence months.2. The separation and identification of pathogenic bacteria We took samples from the onset farm and then separated pathogenic bacteria. We got a total of 87 pathogenic bacterial strains. We found that the main pathogenic bacteria were E. Coli (42.52%), S. aureus (17.05%), S. uberis(9.20%),B. cereus(11.49%), P. aeruginosa(8.04%), S. dysgalactiae(5.14%). The environmental pathogenic bacteria accounted for 71.25%. Proportion of mixed infection was 53.33% and the proportion of single infection was 46.67%. We also found that the infection of aureus was difficult to cure and the infection was recurrent. And what is worse, the cows would become the source of infection. The incidence of E. coli was significant difference (P< 0.05) in different months, with the highest in August.3. Drug sensitive test Taking all the pathogenic bacteria in August for drug sensitive test, all the strains showed different drug resistance. S.aureu showed the strongest resistance and was resistant to most antibiotics tested, with above 90% resistant rate. And what is more, it was mixed resistance. The rest strains were sensitive to clinical commonly used antibiotics and their resistance is poorer. The best drug for treatment of E. coli was ofloxacin and cefepime, for cure of S. aureus was gentamicin, for P. aeruginosa was ceftazidime and amikacin, for B. cereus was erythromycin and imipenem, and for S. uberis was erythromycin and cefotaxime.4. The dynamic analysis of drug resistance In order to know the change of drug resitance of all E. coli within seven months, we observed their drug sensitivity to clinical commonly used drugs continuously. The results showed that their sensitivity to gentamicin was gradually reduce. It meant that the resistance of E. coli had increased. The resistance to ampicillin, cotrimoxazole, penicillin and ofloxacin changed irregularly. The minimum sensitivity to E. coli was clinical commonly used penicillin.5. Comprehensive prevention and treatment of mastitis On the basis of drug sensitive test, we used both Chinese and western medicine to treat clinical mastitis. Comparing single therapy with mixed therapy, we found that the blend of gentamicin and radix isatidis could cure 72.73% clinical mastitis. The cure rate was significantly higher than that of single drug treatment. At the same time, we took preventive measures to reduce the occurrence of mastitis and got the good results.