| Due to its excellent structural characteristics and physical and chemical properties, β-cyclodextrin has become an excellent structural unit for various functional materials. Through the study of vitamin E-β-cyclodextrin inclusion complexes produced the best method, simulate the in vitro release rate research and compare the effect on physiological indexes in SD rats, to find out the effect of vitamin E-β-cyclodextrin inclusion compound relative to vitamin E on SD rats growth. The experimental data were analyzed by Excel 2007 and SPSS 17 software, and the results were found to provide theoretical basis for the preparation of the inclusion compound of vitamin E and added to animal feed in the future.In order to prepare vitamin E-β-cyclodextrin inclusion compound, the experiment was made by microwave method, solution method and ultrasonic method.The inclusion temperature were set as follows:the solution method and microwave method(50 ℃,60 ℃,70 ℃),microwave method were not set because of the uncontrolled temperature s.The inclusion time was set by microwave method(2 min, 4 min, 6 min), solution method(1 h, 2 h, 3 h), ultrasonic method(2 h, 3 h, 4 h).The inclusion ratio were set:microwave method, solution method and ultrasonic method vitamin E and beta cyclodextrin ratio(1:2, 1:3, 1:5). The inclusion compound has been generated by infrared spectroscopy.The results show that the solution method of inclusion ratio 1:3, inclusion time 3 h, inclusion temperature at 70℃ prepared with the best rate of packet to the 72.45%.The results showed that the inclusion compound method of beta cyclodextrin and vitamin E was effective in the present study.In order to detect beta cyclodextrin with vitamin E release rate of inclusion compound, the test by simulating in vivo gastrointestinal fluid environment, respectively in different days,(30 days, 60 days), different packages with simulate of liquid proportion(1:1,1:2,1:3,1:4), different release time(0.5h, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 6h, 8h) and different temperature( room temperature,4℃condition), detected the effect on the release rate.The results show that the release rate at the condition of 4℃was significantly higher than the release rate that of the same time kept at room temperature, so the 4℃ stored are more favorable. Compared to the same temperature, the release rate of 30 days was significantly higher than the 60 days’ release rate, and the shorter the time, the higher the efficiency. When the temperature and time are the same, the release rate of around 70% was significantly higher than that the release rate of about 30% in simulated intestinal conditions, so it can be seen gastric juice has certain effect for the inclusion compound before the inclusion complex was absorpted by small intestinal, and the majority are intestinal release beta, so β- cyclodextrin has significant protective effect on vitamin E. The results of this experiment show that the effect of beta cyclodextrin on vitamin E can be effectively protected by the external environmental factors. In the process of adding the feed, it should be kept in low temperature, and the quality of the feed is better if it can be fed in short time.In the control group、the VE group、Inclusion compound group in the experiment of the antioxidant activity of the rats int, the effect of catalase in rat serum was not significant(P>0.05). The effect of serum malondialdehyde was not significant(P>0.05), The effect of glutathione peroxidasein serum of rats was not significant(P>0.05). But the average value can be obtained from the rat serum catalase inclusion group better than the VE group, the VE group was better than the control group, the serum levels of catalase in the control group、 the VE group、the inclusion compound group had an increasing trend. The average content of malondialdehyde and glutathione peroxidase in rat serum was higher than that in control group compared with VE group.In the measurement of the index of rats, the weight gain of the rats in the control group and the VE group was not significant(P>0.05). In the analysis of serum creatinine in rats, the difference between control group and VE group was not significant(P>0.05), VE group and inclusion group were not significantly different(P>0.05), The control group and the inclusion compound group were significantly different(P<0.05). In the rat serum xanthine oxidase analysis, control group, VE group, inclusion group had no significant difference(P>0.05). In the analysis of serum acetylcholinesterase, control group, VE group, group, the difference was not significant(P>0.05). The serum total cholesterol of rats was analyzed, and the control group, VE group, and the inclusion group were not significantly different(P>0.05). Compared with the VE group, the control group was significantly different in the control group compared with the control group(P<0.05),The VE group and the inclusion group were not significantly different(P>0.05). Group, control group, VE group, the difference was not significant in the serum high density lipoprotein(P>0.05). |
PDF Full Text Request