Effect Of Volatile Substances Of Apple Leaves On Host Selection Behavior Of Lithocolletis Ringoniella Matsumura

Lithocolletis ringoniella Matsumura is a kind of serious damaged pests. Andit damages fruiters of Rosaceae, causing economic losses. Nowadays, the control of L. ringoniellautilize traditional methods, like pesticides, reducing the quality of fruits severely.The insect search for and select appropriate hosts by volatile substances released from plants, which depends on the antennae. The antennae of insects have the function of touch and olfaction, aiding insects to perceive volatile substances. The antennal structures of insect have differences in order to find hosts and fit the external environment, because the kind and the content of volatile substances varies in different plants. The research is analyzed and discussed aboutthe morphology and the kind and distribution of sensilla by SEM, combing the statistics on selection to different processed leaf and the kind, contend of volatile substances determined by gas chromatograph. What we studied can provide theoretical basis about the function of antennal sensilla, the mechanism of host selection and controlling L. ringoniella by volatile substances.The results of the study were as follows:The observation on antennal morphology and sensilla of females and males is used by SEM. Seven kinds of sensilla are discovered in the sensilla of females, including sensilla trichoidea, sensilla chaetica, sensilla basiconica, sensilla coeloconica, sensilla styloconica, sensilla squamiformia, and b?hm bristles, and one more kind of sensilla called malformed sensilla basiconica, is observated in males and females. There is no obvious difference of sensilla between sexes,and the antennal sensilla of L. ringoniella is regularly distributed. A few differences are found in the morphology and distribution of various kind of sensill, some of which have subtypes.The host selection behavior of L. ringoniella is preliminary studied by using Y-tube olfactometer.Results show that the apple leaf volatilesubstances has obvious attraction for L. ringoniella. L. ringoniella has greater selectivity on healthy leaves, followed by the damaged leaf without larva, and finally the damaged leaf.Compositions and relative contents of volatilesubstances of healthy leaves, damaged leaves and damaged leaves without larvae have been analyzed by using gas chromatograph mass spectrometer. There is 21 main volatile substances analyzed in health leaves, which is one volatile substance less than in damaged leaves and four less than in damaged leaves without larvae. 3-Hexen-1-ol, acetate,(Z)-, 3-Hexen-1-ol,(Z)-, Butanoic acid, 3-hexenyl ester,(Z)-, 1-Hexadecanol, à-Farnesene and Styrene exist in apple leaves with three different treatments. It is speculated that these volatilesubstances are the main volatile substances in apple leaves. Volatilesubstances which there is no or very low content in healthy leaves and only exist in the damaged leaves and damaged leaves without larvae are 3-Hexen-1-ol,(E)-,(E)-4,8-Dimethylnona-1,3,7-triene, 9-Octadecene,(E)-, á-Ocimene, 1-Undecanol. It is suggested that these volatilesubstances have the function of driving the moth.