Development And Application Of SSR Markers In Eggplantbased On Transcriptome Sequencing

High generation of self linesS1(10cw60) and S2(RMM620),which were significant different in fruit type,fruit color,and resistant,were sequenced by using Illumina HiSeq2500 platform.The bioinformatic analysis and SSR primers exploitation had been done with sequencing date. Selected primers with high polymorphism after screening were used for genetic diversity analysis in 50 eggplant materials from China, Russia, the United States, Netherlands, Brazil and Franch.The conclusion as follows:1 High generation of self lines 10cw60 and RMM620 were sequenced by using Illumina HiSeq2500 platform, 7374597820 bp and 5538230768 bp raw reads were obtained,respectively.the filtered clean reads were assemblyed with do novo trinity,45404 unigenes were obtained which max length was 13043 bp,min length 301 bp and average length 1055 bp.unigenes blast with Nr database in NCBI,26286 unigenes got function annotation,match ratio was 57.9%. Gene ontology analysis were performed according bioinformation of two materials.2 Software MISA were used to search SSR loci from 45404 unigenes,8316 of SSR loci were obtained,average 5.63 bp got one loci with 18.32% frequency.There had abundant types of SSR loci in eggplant transcriptome which were with distribution from single bases to six repeat bases in large number. The primer were designed from 7048 sequences with 8316 SSR loci by using online software Primer3858 pairs of SSR primer were obtained. Exploit SSR compared with the reported ones in previous, the result showed that there were not repeat in them.858 pairs of SSR primer were screened to detect the effectiveness and polymorphism in 8 eggplant materials with significant different agricultural characteries.3159 pairs of SSR primer with high polymorphism were tested in 50 eggplant materials(23 from Russia,23 from China,1 from the United States,1 from Netherlands,1 from Brazil and1 from Franch.) for genetic diversity analysis. The result showed that PIC of every pair of primer distributed from 0.65-0.11,and 0.19 in average.50 germplasms dendrogram were constructed with UPGMA and SHAN based on genetic similarity coefficient. There were two categories according to Russia and China region except 4 wild relatives,group structural analysis and cluster result were similar,it indicated that the exploit SSR primer were suitable for genetic diversity analysis.