The Effect Of Enhanced UV-B Radiation To Wax And Transcriptome Of Glycyrrhiza Uralensis Fisch Leaf

The increase of UV-B radiation levels in the world has serious harmed to the normal growth and development of plants. The research from different levels and perspectives on the defense and repair mechanisms of plants to UV-B radiation is one of the hot topics in the research of Botany and global agriculture. This experiment was based on the study of glycyrrhiza uralensis, use transcriptome and gas phase mass spectrometry and other means to systematic analysis of the effects of enhanced UV-B radiation on glycyrrhiza uralensis leaf wax layer. The results are as follows:1.With the extension of the time of UV-B radiation,the total wax of glycyrrhiza uralensis leaf become more and more. And the alkanes and fatty acids in wax appeared different degree of increase. The increase of alkanes is the most notable, easters did not change significantly.2. By Illumina sequencing we obtain the transcriptome data that radiation time for 2h,12h,48h, 96h in leaves of Glycyrrhiza uralensis. After assembling were obtained 81158 unigenes sequences, 43902 gene are blasted and annotated,the annotation rate is 54.1%. KOG functional classificate 24953 Unigenes into 25 functions, KEGG database annotation results show that a total of 10570 Unigenes notes to the 123 pathways. And the waxy synthetic pathway related metabolic pathways are fatty acid metabolism pathways of 98 unigenes, fatty acid synthesis pathway of 61 unigenes, fatty acid elongation pathway of 7 unigenes. These genes provide an important foundation for further research on the effects of enhanced UV-B radiation on glycyrrhiza uralensis leaf wax layer.3. By PCA analysis and pairwise difference comparative analysis to the transcriptome data of CK、T2、T12、T48、T96,we found that CK and T2 together as a class, T2 as a class alone, T48 and t96 together as a class. which may imply that in the initial UV-B radiation, UV-B radiation more as a kind of input information to regulate the metabolic pathways of leaves of Glycyrrhiza uralensis Fisch. But with the extension of irradiation time, UV-B radiation become a stress factors from a control signal gradually. Comparison with the control group and the experimental group, we found more and more differences expression genes involved in response to UV-B radiation with the increase of the radiation time and up to 15962 differential genes.4. Further analysis found that UV-B radiation promotes the wax alkane biosynthesis pathway gene expression, inhibit gene expression of biosynthetic pathway of esters and other substances, and to promote the wax to the outside of the cell membrane transport processes. Further analysis showed that 8 non-specific lipid transfer protein (nsLTPs) may be involved in wax transport. And through the RT-qPCR to verify the transcription and expression profile of the data.