| Objective: The different adding dose of Astragalus polysaccharide(APS) was used to pig diet for analyzing the growth performance, slaughter performance, blood biochemical indices and immune factor in different growth stages of growth-finishing pig. Thus it provide the theoretical basis and guiding production for the APS rational utilization and the health of the pig breeding in the pig production.Methods:The 80 health 28d-DLY-piglets of close weight(no significant difference) from the sows of tire time close, litter size stability(12-16) were divided into control group and three experiments(0.15% APS group,0.20% APS group, and 0.30% APS), and each group had four repeat(5 in each group). No APS was added in control group, and 0.15%, 0.20%, 0.30% of the APS were added in experimental group respectively. This test was conducted including preparing period of 10 days and feeding period of 30 days divided into three stages.Pig growth performance was determined and followed to determine the slaughter performance. In the different stages of growth pig serum samples collected respectively, with respect to its different doses of APS on the growth of pig blood biochemical indices and immune factor is analyzed. At the end of each stage, serum samples were collected respectively, and analyzed the effects of different doses of APS on the pig blood biochemical indices and immune factor is.Results:(1) After adding APS, pig slaughter weight, body weight and slaughter rate has an increasing trend(P > 0.05).(2) After adding APS, total protein and globulin in piglet serum had an increasing trend, white balls had a reducing tendency; triglycerides content decreased, and 0.15% group changed significantly(P < 0.05).The total protein and albumin levels were declined in serum of middle-growthing pigs after supplementing APS in the groups of 0.20% and 0.30%(P < 0.05). Triglyceride levels in experimental groups increased, and 0.20%APS group changed significantly(P < 0.05). Total protein content in serum reduced in 0.15% and 0.30% APS groups than other two groups, while 0.20% APS group increased(P > 0.05). After adding the APS, globulin content decreased, the levels of albumin and white ball increased compared with control group in the serum(P > 0.05), and the levels triglycerides and blood glucose increased in 0.15% APS group than control group(P > 0.05).(3) The CD4 levels increased, and CD8 contents decreased in piglets serum in treatment group than control group, and changed significantly in 0.30% APS group(P < 0.05). Compared with control group, the IL-2 levels increased significantly in 0.20% and 0.30% APS groups(P < 0.05), and IL-6 levels increased significantly(P < 0.05) and very significantly(P < 0.01) respectively, and gamma INF content also increased significantly(P < 0.05). The levels of CD4 and CD8 in pig serum increased or decreased significantly in 0.20%or 0.30% APS groups, and the levels of IL-2 and IL-6 increased extremely significant(P < 0.01) or significantly(P < 0.05), and the highest content in the 0.20% APS group. Also gamma INF content in 0.20%APS group increased significantly(P < 0.05). After supplementing APS in pig diet, CD4 levels increased, and changed significantly in 0.20% and 0.30% APS groups(P < 0.01), and CD8 content decreased significantly. In addition, the level of CD8 was lowest in 0.30% APS group, and significant difference with other two experimental groups(P < 0.05). The contents of IL-2 and IL-6 increased in adding APS groups, and changed significantly in the 0.20% APS group(P < 0.05). Gamma INF content also decreased, and changed significantly in the 0.20% and 0.30% APS groups(P < 0.05).Conclusion:(1) Overall considering the growth performance and slaughter performance, the optimum adding amount of APS was 0.20%- 0.30%.(2) After adding different doses of APS, the levels of total protein, albumin,globulin, white balls, triglycerides, and glucose in the serum of different growth stages of serum have different response as the adding dose and different growth stages. The suitable dosage of APS was 0.20% from the overall analysis of biochemical metabolism.(3) Different doses of APS supplemented in the pig diets can improve pig immune factor by improving the levels of CD4, IL-2, IL-6, gamma INF, and proper adding dosage was 0.20%. |
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