Research Of IFITM3Gene Expression In Hepatocellular Carcinoma And Its Relationship With Clinicopathological Features

Research backgroundHCC (Hepatocellular carcinoma)is one of the highest incidence of malignanttumors worldwide. HCC often appears early vascular invasion, capsular invasion，intra-and extrahepatic metastasis. Although we can take number of interventions torecurrence and metastasis of HCC in clinical, such as preoperative percutaneoustranscatheter arterial embolization (TAE) or transcatheter arterial chemoembolization(TACE), postoperative TACE and biological treatment interventions, the overalleffect of these measures are still very limited, the key problem is that we don’t knowthe molecular mechanisms of HCC recurrence clearly and know even less about themolecular regulatory network, so the study of the molecular mechanisms of HCCrecurrence and to take effective measures for the prevention and treatment are majorproblems need to solve in the medical profession.ObjectiveBy detecting the expression of IFITM3gene in hepatocellular carcinoma tissuesand adjacent normal liver tissues to analyze its relationship with clinical features andpathological features of HCC.This aims to clarify the molecular mechanism ofinvasion and metastasis of hepatocellular carcinoma, make it clear of molecularregulatory network of liver cancer invasion and metastasis, provide theoretical basisfor the treatment of liver cancer invasion and metastasis.Methods1. Use western blot to detect the expression of IFITM3in21liver cancertissues and adjacent normal liver tissues storaged in liquid nitrogen from21clinicalcases of liver cancer, analyze the correlation between the IFITM3expression levelsand the clinical characteristics of the patients. Use SPSS19.0to do the statisticalanalysis. Use the t test and the value of P <0.05was considered has statisticallysignificant.2. Use immunohistochemical to detect the expression of IFITM3in17formalin-fixed liver cancer tissues and adjacent liver tissues from the21clinical cases. analyze the correlation between the IFITM3expression levels and thepathological features of the these tissues. Use SPSS19.0to do the statisticalanalysis.Use the χ2test and the value of P <0.05was considered has statisticallysignificant.Results1. Western Blot result shows the expression of IFITM3in liver cancer tissues(mean=1.2399±0.1889) is higher than in adjacent tissues (mean=0.9565±0.0993).there were significant differences between the two(P <0.05).2. Immunohistochemical analysis showed IFITM3protein localized in thecytoplasm, brownish yellow granules. The expression of IFITM3in15specimens of17were positive, the positive rate was88.4%(15/17), in17adjacent normal livertissues, only4cases were positive, the positive rate was23.5%(4/17), there aresignificant differences (P <0.05) between the two, IFITM3gene expression wassignificantly higher in liver cancer tissues.3. The study found that IFITM3expression is higher in cancer specimens frompatients with portal vein tumor thrombus, the result has significant difference (P<0.05), the expression in specimens from patients with TNM stage Ⅲ~Ⅳin HCCis higher than patients with TNM stage I~Ⅱ, the result has significant difference(P <0.05), and the expression of specimens from patients with large tumors is higherthan specimens from patients with small tumors (P <0.05); In poorly differentiatedhepatocellular carcinoma, its expression is also higher compared to welldifferentiation hepatocellular carcinoma (P <0.05)，it has nothing to do with genderand age (P>0.05), and is unrelated to the patient’s bloodAFP levels (P>0.05).ConclusionIFITM3gene expression in hepatocellular carcinoma is significantly higher thanthe adjacent normal liver tissue. IFITM3gene expression is higher in cases withportal vein tumor thrombus, high TNM stage,large tumor size and poordifferentiation.But has nothing to do with gender, age, bloodAFP level.