Application Of Real-time PCR Assay For Detecting Cervical Lesions Patients’ Human Papillomavirus

Cervical cancer is one of the common malignant tumors of female reproductivesystem, is a global public health problem.cases in2008the global new cases ofcervical cancer is about529800,255100deahes,of which85%new cases indeveloping countries.Research suggests that cervical cancer is composed of cervicalintraepithelial neoplasia (CIN) gradually evolved,and cervical cancer’soccurrence,development and comprehensive effects of various risk factors result,thethrough sexual transmission type cervical infection with high-risk human papillomavirus(HR-HPV) is one of the risk factors of cervical cancer and precancerous lesion.HR-HPV infection can be by medical intervention to prevent and cure, therefore,early detection of HR-HPV infection and to take active intervention is the key to theprevention of cervical cancer and precancerous lesions.Objective： Detection of high-risk human papillomavirus DNA real-timefluorescence quantitative PCR method, combined with the papilloma virus infectionin cervical lesions and high-risk human pathological diagnosis,to provide referencedata for early screening of cervical cancer and cervical intraepithelial neoplasia.Methods：63women with cervical diseases for affiliated hospital of qinghaiuniversity from June2011to October2012were slected in this research, high-riskhuman papilloma virus DNA by real-time fluorescent quantitative PCR method in63cases of cervical disease in scecretion, collection on the test results, combined withhigh-risk human infection utervical lesions and high-risk human pathologicaldiagnosis Result:HR-HPV DNA by real-time fluorescent quantitative PCR method in63cases of cervical disease secretion in chronic cervicitis patients, the positive rate ofHPV-16was11.1%,CINⅡ infection in patientswith the positive eate of HPV-16was20%,CIN Ⅲ patients infected with the positive rate of HPV-16was20%, cervicalcancer in different stages HPV-16DNA in plasma were33.3%、58.3%、75%，respectively. the positive rate of HPV-18was16.7%,CINⅡ infection in patientswiththe positive eate of HPV-18was16.6%,CIN Ⅲ patients infected with the positiverate of HPV-18was16.6%, cervical cancer in different stages HPV-18DNA inplasma were15%、16.6%、16.6%，respectively, the difference was ststisticallysignificant(P<0.05).Conclusion：Detection of HPV DNA real-time fluorescence quantitative PCRmethod has good reproducibility, which provided reference for application of themethod to detact HPV DNA for cervical cancer.The occurrence, development andHPV cervical lesions associated with infection, aggravating the degree of cervicallesions, the positive rate of HPV infection significantly increased.