Investiagtion Of Polyfluorinated Chemicals Level In Blood And Semen And Semen Quality For Residents In Pearl Delta Region

This study is targeted to investigate the correlation between concentration ofPolyfluorinated Chemicals (PFCs) exposure level in blood and in semen and semen qualitiesfor residents in the Pearl River Delta (RPD) region in China. Blood samples and semensamples from156infertile volunteers were studied for measures of semen quality and9PFCs.Samples PFCs exposure levels were measured by ASE-LC-MS/MS. Information on the studysubjects’ living habits (such as smoking, drinking) and general information (age,body-mass-index (BMI) and educational background) were also collected.PFCs concentrations in blood detected in this study showed as follows:∑9PFCs:31.81±11.39ng/g, PFBA:3.27±1.92ng/g, PFPrA:0.82±0.71ng/g, PFPeA:3.17±1.81ng/g,PFHxA:4.62±2.81ng/g, PFBS:0.18±0.12ng/g, PFHpA:0.52±0.37ng/g, PFOA:9.06±5.36ng/g, PFHS:4.26±2.81ng/g and PFOS:5.91±4.23. Significant correlations werefound between∑9PFCs concentration in blood with age (r=0.34，p<0.01) and BMI (r=0.41，p<0.01). Smoking habit would increase PFCs accumulateion in blood (∑9PFCs increased2.51ng/g), but that was not very significant with drinking(∑9PFCs increased0.44ng/g). Nocorrelation was found between PFCs concentration in blood and educational background.Pearson statistical result showed significant correlations were found between PFCsconcentration in blood and semen motility grade a (r=-0.18，p<0.05), grade b (r=-0.20，p<0.05)and a+b (r=-0.22，p<0.05). A strong correlation was found between PFCs concentration inblood and semen motility grade d (r=0.25，p<0.01).PFCs concentrations in semen as follows:∑9PFCs：18.90±7.69ng/g, PFBA:3.92±3.46ng/g, PFPrA:1.60±2.29ng/g, PFPeA：2.10±1.47ng/g, PFHxA:4.33±3.41ng/g,PFBS:0.11±0.11ng/g, PFHpA:0.50±0.45ng/g, PFOA:0.91±0.62ng/g, PFHS:0.13±0.11ng/g and PFOS:5.314.71ng/g. Significant correlations were found between∑9PFCsconcentration with age (r=0.32，p<0.01), and BMI (r=0.71，p<0.01). Smoking habit wouldincrease PFCs accumulateion in semen (∑9PFCs increased1.91ng/g), but that was not verysignificant with drinking(∑9PFCs increased0.76ng/g). No correlation was found between PFCs concentration and educational background. Pearson statistical result showed significantcorrelations were found between PFCs concentration in semen and semen motility grade a(r=-0.42，p<0.05), grade b (r=-0.48，p<0.05) and a+b (r=-0.52，p<0.05). A strong correlationwas found between PFCs concentration in semen and semen motility grade d (r=0.54，p<0.01).Comparitive ratio between PFCs concentrations in blood and PFCs concentrations insemen is1.68. Blood-testis barrier (BTB) is a physical barrier to blood vessels and Vasdeferens, it can prevent individuals or cytotoxic substances getting into s deferens. Resultsshow BTB actually played the barrier role in this study. Meanwhile, the study fullfill thedatabase of Pearl River Delta region of human blood and semen levels of perfluorinatedcompounds and hedge exposure to environmental hormones provide a basis to providereference data for the male reproductive health, prenatal and postnatal care has importantsignificance for the region.