Expression Of Mitogen-activated Protein Kinase Signal Pathway And SPAG9in Cervical Cancer Subcutaneous Implanted Tumor Of Pregnant Nude Mouse

ObjectiveTo establish the Cervical Cancer subcutaneous implanted tumor of pregnant nudemouse model, analyze the expression changes of mitogen-activated protein kinase(MAPK) signal pathway and SPAG9and their relationship in implanted tumor tissue.MethodsHealthy female nude mice were randomly divided into two groups after successfulcopulation. Both the experimental and control groups (n=10each) received rightaxillary subcutaneous inoculations: recovery human cervical cancer HeLa cells in theexperimental group, and saline in an equal volume in the control group. Both theweight of the nude mice and tumor volume were measured. The mice were euthanizedand the tumor was completely removed14days after inoculation. Hematoxylin andeosin staining was used to observe the tumor cells morphology. Western blot was usedto detect the activity of ERK1/2, JNK1/2, P38phosphorylated protein of the MAPKsignal transduction pathway and SPAG9protein in implanted tissue in both groups.Real-time fluorescent quantitative PCR was used to detect SPAG9mRNA expression inimplanted tissue in both groups.Result1.Human Cervical Cancer HeLa cell line single cell suspension was successfullyinoculated by right axillary subcutaneous inoculation in pregnant nude mouse with anachievement ratio of100%.2.Mean p-ERK1/2, p-JNK1/2and p-P38activity was significantly increased in thesubcutaneously implanted tumor of the experimental group as compared to the controlgroup.3.Mean p-ERK1/2activity correlated positively with p-JNK1/2in the implantedtumor of the experimental group, but had no correlation with p-P38.4.Mean SPAG9mRNA and SPAG9protein expression was significantly increased in the subcutaneously implanted tumor of the experimental group as compared to thecontrol group.5.Mean SPAG9mRNA and SPAG9protein expression correlated positivelywith p-ERK1/2, p-JNK1/2activity respectively, and negatively with p-P38.Conclusion1.The Cervical Cancer subcutaneous implanted tumor of pregnant nude mousemodel can be established successfully by subcutaneous inoculation of HeLa cell onpregnant nude mouse.2.SPAG9may take part in the regulation of MAPK signal pathway primarilythrough the JNK pathway.3.Increased expression of SPAG9may activate the MAPK signal pathway andpromote the development of Cervical Cancer in pregnant nude mice.