| Objective:To screen for microRNAs whose expression varies in Hashimoto thyroiditis (HT) associated with papillary thyroid carcinoma (PTC), and to predict target genes of the detected microRNAs to investigate possible mechanisms of their effects.Methods:RNA was extracted from fresh tissues derived from HT/PTC (n=3), and microRNA expression was examined by microarray assays, with tissues from nodular goiter (NG) used as controls (n=3). Real-time quantitative PCR was employed to verify the microarray results. Prediction of target genes of microRNAs and construction of regulatory network was performed using a number of prediction softwares. Expression of the predicted target genes was examined by microarray assays. Subsequently, microRNA-130a (miR-130a) expression was examined in tissues derived from HT/PTC, follicular thyroid carcinoma (FTC) and medullary thyroid carcinoma (MTC)(n=10for each group) using real-time quantitative PCR, and variation of miR-130a in different types of thyroid cancer was determined.Results:Compared to the controls, miR-130a expression was reduced by five folds in HT/PTC (P<0.05). Consistently, a2.25-fold reduction in miR-130a expression was observed in HT/PTC as determined by real-time quantitative PCR. On the other hand, HT/PTC induced a16.9-fold and17.8-fold elevation in WNT10A expression, the predicted target gene of miR-130a, as determined by microarray assay and real-time quantitative PCR respectively. Subsequent experiments showed that HT/PTC, FTC and MTC induced approximately2-,6-and10-fold reduction in miR-130a expression (P<0.01) respectively.Conclusion:miR-130a might be involved in the pathogenesis of HT/PTC via regulation of the WNT10A gene. A correlation was observed between the reduction in miR-130a expression and the prognosis of different types of thyroid cancers, suggesting that miR-130a down-regulation might contribute to different types of thyroid cancer and the progression. |
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