| Research background:Cerebrovascular disease is a group diseases influenced by multi-factors, and the gene polymorphism is possibly one of pathogenesis. Nitric oxide(Nitric oxide, NO) is a kind of important active substances, found in pathological physiological process, and involved in a variety of NO in the cardiovascular system is one of the important protective factor. It is adjusted by three kinds of nitric oxide synthase. In recent years,some research indicated the endothelial nitric oxide synthase (eNOS) polymorphism and ischemic cerebrovascular disease occurrence related. The present study was carried out to evaluate the relationship between G894T polymorphism of endothelial nitric oxide synthase and patients with ischemic cerebrovascular disease in Wa people of Yunnan.Research approach:①We investigated48unrelated patients of Wa nationality with ICD (25men and23women).The age of onset concentrates between43years old and79years old.All patients were selected from the department of neurology in4hospitals of Lincang.and all cases underwent head CT scan or MRI.The50unrelated controls of the Wa nationality aged between40to80years,were selected from the department who underwent regular check-up examinarions(24males and26females);The controls had no history of ICD. There were51total patients of Han with ICD including24males and27females The age of onset concentrates between40years old and77years old.②eNOS G894T gene polymorphism were analyzed by polymerase chain reaction (PCR).③The samples were positive and negative tested with gene sequencing.④The results were compared between the patient group of Wa and control group of Wa, The patient group of Wa and patient group of Han,the male group and female group,the patients with hypertension group and normal blood pressure group, the patients with diabetes group and normal blood glucose group,the patients with dyslipidemia and lipids in normal group.⑤Count data were performed by t-test.Genotype and allele frequencies were compared using the χ2analysis.We calculated the genotypic odds ratio(OR) and their95%eonfidence interveal(CI)for patients with ICD.Hardy-weinberg equilibrium was confirmed with the χ2test.All stastical analysis were performed with SPSS13.0software.Results:There were three kinds of genetypes of G894T in eNOS polylnorphism,namely GT heterozygous,GG homozygous,TT homozygous,were identified.The frequencies of the three genetypes were as follows:GG type32(66.7%)、GT type12(25.0%)、TT type4(8.3%) in patient group,GG type28(56.0%)、GT type14(28.0%)、TT type8(16.0%) in control group and GG type37(72.5%). GT type12(23.5%)、TT type2(4.0%) in Han group.The frequencies of the two alleles were as follows:G allele79.2%、T allele20.8%in patient group,G allele70.0%、T allele30.0%in control group and G allele84.3%、T allele15.7%in Han group.We found that the frequency of GT+TT genotype and T allele in ischemic stroke patients of Wa nationality has increased than the control group of Wa people.There’s no significant difference between these2groups(χ2=0.020, P>0.05;χ2=0.103, P>0.05).And the frequency of GT+TT genotype and T allele in ischemic stroke patients of Wa nationality has increased than the control group of Han people with ischemic stroke.There’s no significant difference between these2groups (χ2=0.405, P>0.05;χ2=0.881, P>0.05).The frequencies of the three genotypes were as follows:GG type19(76.0%)、GT type5(20.0%)、TT type1(4.0%) in men of Wa patient group,and GG type13(56.5%)、 GT type7(30.4%)、TT type3(13.1%) in women of Wa patient group.In men,G:T frequency was0.86:0.14;in women,G:T frequency was0.717:0.283.There was no difference in the frequency of the GT+TT genotype between men and women in Wa patient group (24.0%versus33.5%; P>0.05) or in T allele frequency (14%versus28.3%;P>0.05).There was a total of39cases of cerebrovascular disease patients with primary hypertension in the WA nationality group, and genome into28cases of GG genotype (71.8%),8cases of GT genotype (20.5%),3cases of TT genotype (7.7%); G allele accounted for82.1%,T allele accounted for17.9%.And the normal blood press group consisted of9patients, including4cases of GG genotype (44.4%),4cases of GT genotype (44.4%),1cases of TT genotype (11.2%); G allele accounted for66.7%, T allele accounted for33.3%.We found that there’s not a significant difference in the genotype distribution and allle frequency of G894T comparing patients with and without primary hypertension in group. The cerebrovascular disease of Wa nationality group with diabetes consisted of7patients,including3cases of GG genotype (42.8%),2cases of GT genotype (28.60%),2cases of TT genotype (28.60%); G allele accounted for57.1%, T allele accounted for42.9%.And the other group consisted of41patients, including29cases of GG genotype (70.7%),10cases of GT genotype (24.4%),2cases of TT genotype (4.9%); G allele accounted for82.9%, T allele accounted for17.1%,There was no difference in the frequency of the GT+TT genotype with diabetes and without diabetes in Wa patient group (57.20%versus29.3%; P>0.05) or in T allele frequency (42.9versus17.1%; P>0.05. The patients with dyslipidemia consisted of26patients,including17cases of GG genotype (65.4%),7cases of GT genotype (26.9%),2cases of TT genotype (7.7%); G allele accounted for78.8%, T allele accounted for21.2%,And the normal LDL-C patients are22cases, including15cases of GG genotype (68.2%),5cases of GT genotype (22.7%),2cases of TT genotype (9.0%); T allele accounted for20.5%.The allele and genotype frequencies of eNOS G894T did not differ significantly in the patient with dyslipidemia compared to the patient without dyslipidemia.Conclusion:1. Wa patients with ischemic cerebral infarction with GT、TT genotype and T allele frequencies compared to the control group, and Han population cerebral infarction and most reports in the Han population has increased, subject to further expand the sample size confirmed.2.The frequency GT、TT genotype and T allele frequencies in the Wa nationality groups of male and female showed no significant differences in distribution.3. Wa patients with primary hypertension in cerebral infarction patients with GT、TT genotype and T allele frequencies compared to the cerebral infarction patients without primary hypertension in Wa nationality group has decreased.Wa nationality with diabetes group in cerebral infarction patients with GT、TT genotype and T allele frequencies compared to the normal blood glucose group has increased significantly. The eNOS gene G894T polymorphism of WA nationality and blood glucose、blood pressure、lipids did not significantly associated. |
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