Dynamic Observation Of Early Fatty Liver In Rats Plasma CD62p Expression And Its Relationship With Blood Stasis

objective:The research on the specificity of the platelet activation index CD62p as breakthrough point, early fatty liver rat model was established, dynamic observation of fatty sex early fatty liver in rats plasma CD62p expression, to explore the early fatty liver, the relationship between platelet activation and blood stasis. From the perspective of "blood stasis" to clarify the early fatty liver of traditional Chinese law, which provides the theoretical and experimental study of TCM clinical treatment of fatty liver.Methods:160rats, randomly divided into5groups。Normal group A50, normal group B only20, model group A only50, model group B20, medicine group20. At4.6.8weekend kill normal group A, model group A10rats seperately. At8weekend began to normal group, model group B saline lavage and medication group of diaphragmatic past by stasis soup to fill the stomach, gastric volume of1ml/200g,1times a day. Profile control in10.12weekend kill every10groups of rats. In weight in rats,5time points after extraction of rat blood profile control after liver tissue, said the rat liver wet weight, liverbody ratio index calculation. Do the liver pathological observation judgment model rat hepatocyte adipose change copy success. Enzyme linked immunosorbent (ELISA) experiment testing5points in the rat plasma soluble P-choose the content of element (CD62p), at the same time do serum alanine aminotransferase (ALT), blood lipid, liver fat, platelet aggregation rate was measured.Results:1. The general situation:four weeks before the normal group and model group rats eating is normal, active model group.5weeks after the model set of food intake and weight gain faster, activity less than normal group, the colour slants dark, runny stool, some rats loose stools. model group weight growth after the8weekend, the colour is dark, action is relatively slow, observed group color returns to normal. Body weight of rats increased continuously,8weekend model group rats weight is higher than normal group (P<0.05).8weekend model of rat liver wet weight is higher than normal group (P<0.05),12weekend increased liver wet weight of model group rats is more obvious.2.Morphological changes:4weekend there was no significant alteration of the liver cells, a small amount of cells appeared6weekend steatosis,8weekend severe steatosis model group cells, liver cells swelling, turn round, fat vacuoles of cytoplasm in size. That early fatty liver model copy success.10weeks,12weeks the pathologic features more obvious. Compared with model group under the diaphragm by stasis soup group,10.12weeks pathological changes significantly mitigated.3. Biochemical indexes:the rat liver fat content of4.6.8.10.12weekends model group was higher than normal group (P<0.01);10and12weekend observed group was lower than those of model group (P<0.05).Model group rats blood lipid with the build time increased. Serum of rats model group higher than normal group at the6.8.10.12weekend, the difference was statistically significant (P<0.05). Diaphragmatic under10weekend by stasis soup group was lower than those of model group, the difference was statistically significant (P<0.05); Diaphragmatic under12weekend by stasis soup group was lower than those of model group, the difference was statistically significant (P<0.05).Model group rats serum Alt along with time increased.6,12,8,10weekend weekend weekend weekend high model group compared with normal group, the difference was statistically significant (P<0.05). Diaphragmatic under10weekend by stasis soup group was lower than those of model group, the difference was statistically significant (P<0.05),12weekends observed group was lower than those of model group, the difference was statistically significant (P<0.05). Rate of platelet aggregation in the rat model group along with increasing time.10and12weekend high model group compared with normal group, the difference was statistically significant (P<0.05).10and12weekend under the diaphragm by stasis soup group was lower than those of model group, the difference was statistically significant (P<0.05).Rat plasma CD62p4.6.10.12weekend high model group compared with normal group, the difference was statistically significant (P<0.05); model group compared with normal group, the difference was statistically significant (P<0.01) at the8weekend;10and12weekend under the diaphragm by stasis soup group is lower than model group, the difference was statistically significant (P<0.01).Conclusion:1.Fatty liver rat model (that is the early fatty liver rats model) copied successfully at the eighth weekend.2.Early fatty liver model of rats induced by high-fat feed plasma CD62p expression increased along with the increase of liver steatosis.3.The early fatty liver model of rats induced by high-fat feed platelet aggregation rate increased along with the increase of liver steatosis, suggests that early fatty liver may be blood stasis. Under the diaphragm by the content of platelet aggregation rate after stasis soup significantly reduced, bolstered by the hypothesis that early fatty liver may be blood stasis.4.Early fatty liver model of rats with diaphragmatic down by stasis soup after lavage, blood fat, fat liver, platelet aggregation rate significantly decreased, liver function damage to reverse, plasma CD62p expression is suppressed, suggests promoting blood circulation to remove blood stasis syndromes of early treatment of fatty liver have good curative effect. Early clinical blood circulation by removing blood stasis method can be used in treatment of fatty liver.