The Study On The Detection Of GDF3Combined CA125, CA15-3and CEA In Diagnosis Of Breast Cancer

Breast cancer is one of the most common malignant tumor of womentoday, incidence of a disease in the first of all female malignant tumors. Nowbreast cancer has become the first big disease cancer related death in women,Female breast cancer mortality is increasing year by year.And breast canceritself is one of the best solid tumors of the curative effect. So early diagnosisand treatment to reduce the mortality of the patients with breast cancer,improve the patient’s quality of life and prolong the living time is a veryimportant role. Tumor markers is widely used in the usual medical importantmeans of early detection of breast cancer. It exists in the blood, cells, tissues,body fluids, and many other places can be effectively confirmed the existenceof the tumor and growth. Its diagnosis and differential diagnosis of tumor andcurative effect evaluation and recovery situation is of great significance.Current clinical commonly used serum markers were carcinoembryonicantigen (CEA) and carbohydrate antigen125(CA125) and carbohydrateantigen15-3(CA15-3), a combination of these three tumor markers detectiondiagnosis to assist clinical early screening.Objective:This study was to explore human growth differentiation factor (GDF3)serum index for the diagnosis of breast cancer, significance and effect. Anddiscussed the CEA, CA15-3, CA125and GDF3single independent inspectionand joint the meaning and value of diagnosis of breast cancer.Tumor markersjoint inspection result analysis, confirmed the tumor markers joint inspectionthe sensitivity and specificity were higher than that of the single parametermonitoring, Can increase the accuracy in early diagnosis of breast cancer, ishelpful to early diagnosis of breast cancer. Methods:1. Object of study:(1)Breast cancer group is in November2012-January2014in our hospital during the period of oncology department in the hospital,the imaging ultrasound, mammary gland molybdenum target inspection andpostoperative pathological examination and verification,52cases of womenwith breast cancer, their age is42years old to75years old, with an average of54.2years.(2)Benign breast disease group is in the same period in our hospitalin the hospital, according to their sick after inspection and ultrasonic andblood, and postoperative pathological examination confirmed the40femalepatients with benign breast disease of breast cancer, including31patients withmammary gland fibroma, and6patients with breast cystic hyperplasia,3patients with lipoma, their age is39-71, with an average of52.8years.(3)Normal control group with40people, who were examined in our hospitalmedical center of women’s health check-up, centerless, important organs suchas liver, lung, and kidney disease, age, at the age of31-78with an average of55.1years.2.Specimen collection and processing: Acquisition research object fastingvenous blood3mL(if you want to do breast surgery patients, both inpreoperative acquisition), let stand20min,15min, fully carries on thecentrifugal and timely detection. Using enzyme-linked immunosorbent(ELISA) to determine GDF3, instrument we chose to use BioRad BioRad450enzyme standard instrument company.The determination of CA125, CA15-3and CEA choose electrochemiluminescence immunoassay, instrument withroche company makes first EleesysE601automatic electrochemicalluminescence immunity analyzer.All reagents for instrument reagent,alloperations must be in accordance with the instrument and reagents andequipment on the instructions.3.Statistical methods: Choose SPSS17.0statistical software for allmeasurement data statistics processing.measurement data determinationresults with x±s. We adopt the method of variance analysis is comparedbetween group, if need to compute data and speed,1we adopt the method of chi-square.Sensitivity and application, specific degrees and the accuracy of/(a+d)/(a+b+c+d)]. Respectively to evaluate the index for the diagnosis ofbreast cancer and the effect of joint in the diagnosis of breast cancer,inspection standard if P <0.05, think they conform with the principle ofstatistics.Results:1. Breast cancer, Benign breast disease group and healthy groupGDF3,CA125,CA15-3and CEA comparison:Breast cancer,benign breast disease group and healthy group, serumlevels of index GDF3is respectively:148.8±19.9pg/ml,95.2±13.7pg/mland92.9±18.4pg/ml. Breast cancer patients serum level of GDF3comparedwith benign breast disease group and healthy group, P <0.05, differences inaccordance with the requirements of the statistical standard. Benign breastdisease group compared with the healthy group,serum index GDF3P>0.05,which do not require statistical differences.Breast cancer and benign breastdisease group and healthy group serum index of CA15-3levels are:49.6±15.3U/ml,19.1±8.5U/ml,18.7±5.2U/ml. Breast cancer group CA15-3levels of serum markers and benign breast disease group and health controlgroup, the difference has statistical significance.Benign breast disease groupof serum markers of CA15-3levels compared with healthy group, is not inconformity with the statistical differences. Breast cancer and mammary glanddiseases group and healthy group of serum CA125level:51.5±17.2U/ml,22.7±6.6U/ml,19.9±4.8U/ml. Breast cancer serum CA125level andbenign breast disease group compared with the healthy group, accord with therequirement of statistical difference. Benign breast disease group comparedwith healthy group, the serum levels of CA125is not in conformity with thestatistical differences. Group of breast cancer and breast benign disease groupand healthy group of serum CEA levels:16.4±8.1ng/ml,3.2±1.9ng/ml,2.5±1.6ng/ml. Breast cancer group serum CEA levels compared with benignbreast disease group and healthy group, in accordance with statisticaldifferences. Benign breast disease group compared with healthy group, the serum CEA level is not in conformity with the statistically significantdifference.2. GDF3, CA125, CA15-3and CEA in four separate serum markersdetection accuracy and sensitivity, specific and comparison:GDF3sensitivity, specific degrees and accuracy in the order:15.4%(8/52),77.5%(31/40),42.4%(39/92); CA125sensitivity, specific degrees andaccuracy in the order:17.3%(9/52),80.0%(32/40),44.6%(41/92); CA15-3sensitivity, specific degrees and accuracy in the order:32.7%(17/52),87.5%(35/40),56.5%(52/92); The CEA sensitivity, specific degrees and accuracy inthe order:26.9%(14/52),97.5%(39/40),57.6%(53/92). CA15-3and CEAseparate detection of breast cancer, the sensitivity of the specific degrees andaccuracy are better than that of CA125and GDF3, accord with statisticaldifference (P <0.05).3. GDF3, CA125, CA15-3and CEA joint four markers detectionaccuracy and sensitivity, specific and comparison:Four kinds of serum index both joint its sensitivity are better than thesingle parameter determination, specific degrees compared with the singlemeasurement is a little lower, but the accuracy are improved obviously. TheCA15-3and CEA joint highest sensitivity and accuracy, and46.1%and64.1%.Three indicators and four indicators combined detection compared withseparate indicators and two both detection sensitivity and accuracy to riseagain. Sensitivity and accuracy of the best three indicators of joint detection ofCA125+CA15-3+CEA combination. And GDF3, CA125, CA15-3and CEAfour indicators coalition detection sensitivity was61.5%, its specific degree72.5%,66.3%accuracy. So together, combined the four best index of serum inthe diagnosis of breast cancer, but the specific degree is relatively low, soshould cooperate with other inspection method on clinical application, toavoid the misdiagnosis.Conclusion:1．This experiment by means of52cases of breast cancer patients serummarkers CEA, CA125, CA153and GDF3level testing,results showed that the four indicators for the diagnosis of breast cancer has certain significance ofbreast cancer patients serum of four indicators were significantly higher thanthat of benign breast diseases group and healthy group, the difference wasstatistically significant.Of breast cancer patients serum GDF3is obtained byexperiment compared with benign breast disease group and healthy group, thedifference was statistically significant.2．GDF3, CA125, CA15-3and CEA four serum marker detection resultscompare alone, its sensitivity in the order: CA15-3> CEA> CA125> GDF3,followed by its specific degrees: CEA> CA15-3> CA125> GDF3, itsaccuracy in the order: the CEA> CA15-3> CA125> GDF3.Comprehensiveanalysis: the four kinds of serum index detection alone diagnostic accuracy ofbreast cancer susceptibility, specific degrees and CA15-3and CEA is best.3．About GDF3, CA125, CA15-3and CEA detection alone, comparedwith three indicators and four indicators joint detection is two separateindicators and the increase of detection sensitivity to some extent, but thediagnosis of specific degrees compared with single detection and two two jointdecreased obviously, and significantly higher accuracy, the joint detectionGDF3, CA125, CA15-3and CEA four serum index for breast cancer diagnosisaccuracy rate is highest.The clinical practical value remains to be furtherdiscussed.