The Study On The Detection Of VEGF,CEA And NSE Combined In The Diagnosis Of Lung Cancer

Objective:This study used VEGF, and CEA, NSE joint detection, and to investigatethe three (VEGF, CEA, NSE) diagnostic value of combined detection in lungcancer.Tumor markers joint inspection results,confirmed the tumor markersjoint inspection the sensitivity and specificity were higher than that of the sin-gle parameter monitoring,could enhance the positive detection rate ofcarcinoma of the lungs.Tumor markers detection together is not a simpleaddition of detection efficiency, detection efficiency could be greatlyincreased by joint detection after falling.The purpose of this article is toprovide a used in the theory of the early clinical diagnosis and treatment oflung cancer.Methods:1.Object of study (1)Lung cancer group:for our hospital betweenSeptember2010and In20135menstrual pathological diagnosis confirmed85cases of patients with lung cancer,31cases of lung adenocarcinoma and squa-mous cell carcinoma of the lung43cases,6cases of large cell lung cancer,small cell lung cancer in5cases.28cases (57year old male and female,44to73years old, average55.2.Adopt the internationally used TNM stagingmethods including14cases of phase I~Ⅱ Ⅲ period30cases,41casesⅣperiod.(2)Benign disease group of40patients with lung benign disease for thesame period, including4patients with bronchiectasis disease, chronicobstructive pulmonary disease patients in5cases,10cases of pulmonarytuberculosis patients,10patients with pneumonia, chronic bronchitis,5cases,5cases with lung abscess, and other5cases; Occurrences for25cases, maleof female man presented for15patients, average age between40to68yearsold,average58.9years old.(3)Health groups:40cases for the same period in our hospital physical examination, the male27cases,13cases of female, aged35to76, the average52.6. Gender, age and other data of three groups ofindividuals by SPSS17.0analysis results P>0.05, the difference of the groupare compared between groups.2.Specimen collection and processing:the research objects are taken on anempty stomach elbow venous blood3ml,Quiet place at room temperatureafter20min to3000RPM, centrifugal extraction of the upper serum (avoiddrew blood cells), placed in-20℃low temperature refrigerator storage andunder test. VEGF using enzyme-linked immunosorbent method, instrument B-ioRad450enzyme mark of BioRad company in the United States. CEA, NSEconcentration detection method for electrochemical luminescenceimmunoassay, instrument for Roche (Switzerland) based automaticelectrochemical luminescence immunity analyzer (type of instrument forRoche EleesysE601).All reagents, reagent instrument for all operations in stri-ct accordance with the instrument and reagents equipment instructions.3Statistical methods use SPSS17.0as the statistical software for statistic-al analysis of the present study, the mean+/-standard deviation according tomeasurement data, choose the single factor analysis of variance between mult-multiple sets of comparison, with SNK test compared between two groups,using t test to carry on the contrast between the two groups, the count dataexpressed as a percentage, with chi-square (chi-square test comparing betweengroups, P <0.05for the difference.Results:1.Between the three groups (lung cancer group, benign disease group andhealth control group) compare: VEGF lung cancer group, serum levels ofNSE and CEA with other two groups (lung benign disease group and healthcontrol group)(P <0.05). Comparison of benign diseases group and healthycontrols:the content of serum VEGF, NSE and CEA P>0.05.VEGF, NSEand CEA joint detection:separate detection of serum VEGF, NSE andCEA,Sensitivity to40.0%,44.7%and42.3%respectively; Tumor markersbetween two joint detection sensitivity: the CEA+VEGF was51.7%, the NSE+VEGF was57.6%, the CEA+NSE was63.5%, the combineddetection sen-sitivity was69.4%. VEGF, NSE and CEA joint detection specificity decreased,but the precision is greatly in.creased by64.8%.2.Three indicators joint detection rate of the single parameter detectionhas improved significantly.Positive rate is Ⅰ~Ⅱperiod42.9%respectively(P <0.05), Ⅲ~Ⅳ period by83.1%(P <0.05),the results showed that the ea-early tumor.of lung.cancer early detection index is less than the speed of theperiod.Joint detection indicators in Ⅰ~Ⅱperiod lung cancer is only42.9%positive rate is not too ideal, but a separate detection of three tumor markerspositive rate has been increased greatly.Separate testing each tumor markers,regardless of stage Ⅰ~Ⅱ or Ⅲ~Ⅳlung cancer group, serum NSE positiverate is higher,28.6%(P <0.05),53.5%(P>0.05). Ⅲ~Ⅳ stage positive ratefrom high to low in turn is the NSE> CEA> VEGF;The CEA, VEGF, NSEtwo joint detection stage Ⅰ~Ⅱlung cancer:CEA+VEGF, NSE+VEGF,CEA, NSE positive rate was28.6%,35.7%and28.6%,respectively,combination of NSE+VEGF positive rate is highest (P <0.05);the CEA, VEGF, NSE two joint detection stage Ⅲ~Ⅳlungcancer:CEA+VEGF, NSE+VEGF, CEA, NSE positive rate were60.5%,62.4%,71.8%, CEA+NSE combination.was.maximum.（P <0.05）.3. Squamous carcinoma group compared to the content of serum NSEand CEA in adenocarcinoma of NSE and CEA levels in the high(P <0.05),onthe contrary, Serum VEGF level in no difference(P>0.05) between the othertwo groups(squamous cell carcinoma of the lung,lung.adenocarcinoma.group).Serum CEA, VEGF, NSE levels.in adenocarcinoma.of lung.And com-parison between.squamous cell.carcinoma of.the lung:.adenocarinoma oflung and lsquamous cell.carcinoma.of the lung patients.serum.tumor markersCEA and NSE levels are different (P <0.05),Small cell lung.cancer patientsserum NSE levels significantly.higher than that of adenocarcinoma.and squa-mous cell.carcinomas.of the NSE levels (P <0.05).4.Tumor.markers.of.the.three.(CEA, NSE and VEGF) in serum content increasing trend along with the tumor stage (P <0.05)..5.Between the.two groups (squamous.cell.carcinoma.of the.lung, lungadenocarcinoma group) three tumor markers (CEA, VEGF, NSE) sensitivityof comparison:lung adenocarcinoma group CEA sensitivity compared with thetwo tumor markers(VEGF, NSE) in lung adenocarcinoma was different (P <0.05);Lung adenocarcinoma group two tumor markers (NSE, VEGF) thesensitivity of the detection of lung cancer than: VEGF detection alone (P <0.05), the NSE of the same (P <0.05).;Two tumor markers (CEA, NSE) todetect lung adenocarcinoma compared.with.CEA single. detection sensitivityimproved (P <0.05), NSE.of.the same;two..tumor..markers..(CEA., NSE)joint detection combined lung squamous carcinoma group compared withmarkers(CEA, NSE) of.the.two single detection..sensitivity..improved(P <0.05);two groups of..the..three..tumor..markers.(VEGF, NSE,CEA.) jointdetection sensitivity.compared.with.CEA,.VEGF, NSE separate detectionsensitivity improved (P <0.05).Conclusion:1.Tumor markers of the three(VEGF.NSE.CEA) joint detection can to acertain extent improve the positive rate of lung cancer diagnosis.2.Squamous carcinoma group, serum NSE and CEA levels were higherthan NSE and CEA in adenocarcinoma group.3.Tumor markers of the three(VEGF, NSE and CEA) in serum contentwith an increasing trend(P <0.05) of cancer stage.