Therapeutical Effects Of Total Flavonoid Of Pteris Multifida In Nonbacterial Prostatitis Rats And Its Safety Evaluation

Objective: Research the therapeutical effects of total flavonoid of Pteris multifida(TFPM) in nonbacterial prostatitis rats and the possible mechanism, the satety of TFPM was also been evaluated.Methods: Intraperitoneal injection of the rat prostate protein purification liquid mixture with immune adjuvant for the preparation of experimental rat model of chronic nonbacterial prostatitis, The rats were randomly divided into 6 groups, the blank control group, model group, TFPM group(high, medium, low dose), prostat group, were fed with relevant drugs and distilled water respectively for 30 days. Then estimated the body weight, immune organs weight and prostate weight, observed the pathological changes at the prostate tissues, evaluated the level of IL-8, IL-10 and TNF-a in the serum, measured the activety of SOD and level of MDA in the prostatic tissue. SD rats were randomly divided into 4 groups for safety evaluation, the blank control group, TFPM group(high, medium, low dose). The rats were fed with relevant drugs and normal saline respectively for 3 months, during the study ordinary physiological indexs such as weight and waste was observed, then detect the hematology, blood biochemistry, organ weight and tissue pathological indicators respectively after post-processing rats.Results: TFPM can not reduce the rats weight and prostate wet weight. Compared with rats in model group, high dose TFPM treated groups had remarkably lower spleen and thymus gland wet weight, the difference between groups was statistically significant(P<0.05). TFPM can obviously improved the prostate tissue in rat models of structure damage and interstitial edema, lower levels of inflammatory cells infiltration, the high dose group improved the most obviously. Compared with rats in the blank control group, the model group had remarkably higher IL-8, IL-10 and TNF-a levels, the difference between groups was statistically significant(P<0.05). Compared with rats in the model group, the serum levels of IL-8 and IL-10 in high dose group was obviously depressed by TFPM, the difference between groups was statistically significant(P<0.05), however the serum levels of TNF-a was reduced remarkably(P<0.01); The middle dose group had a similar reduce about the levels of IL-8 and TNF-α compared with model group(P<0.05). Compared with blank control group, the model group had a increased MDA levels and decreased SOD activety. All the three dose of TFPM decreased the content of MDA and showed no significant effect on the activety of SOD in prostate tissue. I n the study of safety evaluation, during the experiment period the commonly physiological indexes such as weight, waste, food and water consumption of SD rats had no significant change. The hematology and blood biochemistry index of each dose group rats did not see obvious change. Individual organs weight and index was statistically diference compared with blank control group, but did not see a dose-response relationship. Meanwhile, the histopathological results indicate no obvious pathological changes about those individual organs.Conclusion: This study showed that intraperitoneal injection of the rat prostate protein purification liquid mixture with immune adjuvant propionate could replicate of the model of chronic nonbacterial prostatitis in rats successful. TFPM could significantly improved the prostate tissue in rat models of structure damage and interstitial edema. The mechanism might be TFPM could improved the immune function, balanced the production of inflammatory factors and adjusted the balance on oxidation reaction by scavenging oxygen free radicals. Meanwhile, the safety evaluation showed that the TFPM has good performance on safety.