| Objective: Through the clinical observation of high, low dose GTW, CTX on malerats testis, liver and so on, to discuss the effects of male rats,reproductive function,liver function and other effects, differences and their reversibility of any difference.Methods:200male SD rats,3weeks old,were randomly divided into5groups, theblank group (n=40), GTW high dose group (high GTW group,n=40), GTW low dosegroup (low GTW group,n=40), CTX high dose group (high CTX group,n=40), CTXlow dose group (low CTX group,n=40). For8weeks to the rats lavage, one time aday. GTW high and low dose groups were given respectively GTW from mouth by12mg/(kg·d)、6mg/(kg·d), high and low dose group of CTX are filled CTX inaccordance with the12mg/(kg·d),6mg/(kg·d), the blank group releases the samevolume of0.5%CMC-Na to fill the stomach. The last administration over24hours,each group were randomly selected10rats, take the venous blood for the number ofwhite blood cell (WBC), alanine aminotransferase (ALT), aspartate aminotransferase(AST) and serum testosterone (T), take testis, liver for calculation of their indexes,observing the pathological changes of testis and liver tissue. And the rest of the malerats were fed in different cages,1per cage, according to the proportion of1:2into2adult female rats about10weeks old, cage feeding. After the female rats pregnancy,part of the male rats removed were anesthetized, taking blood, liver, testis for testingand observing the above indicators, detailing records of each cage female rats time topregnancy, litter size and conception rate.Results:1.The number of white blood cells: At the end of the last administration to fill thestomach, order in terms of the number of white blood cells was follows: low GTWgroup>high GTW group>control group>high CTX group>low CTX group. Comparedto low CTX group, the control group and low GTW group had significant differences(P<0.05), high CTX group respectively compared with low CTX group and high GTWgroup, there were no significant differences (P>0.05), and obvious differences were not found between low and high GTW groups (P>0.05); after caging, the number ofwhite blood cells of all groups were close, there were no significant changes amonggroups (P>0.05).2.Changes of liver function: Results from the ALT, whether the last stomached or aftercaging, control group was the lowest, but among the groups showed no statisticalsignificance (P>0.05). From the AST results, at the end of the lavage, compared to theblank group, medicined groups increased, but there were no significant differencesamong the groups (P>0.05); after stopping medication, high GTW group increasedsignificantly compared with the control group, there were significant differences inthe statistical analysis (P<0.05), but low GTW group respectively compared with lowCTX group and high GTW group, there were no significant differences (P>0.05).3.Changes in testosterone levels: During the two periods, compared with controlgroup, medicined groups of testosterone concentrations increased to varying degreesduring the same period, low CTX group was the highest. Low CTX group respectivelycompared with low GTW group and blank group of the same period, there weresignificant differences (P<0.05), while there were no statistical significances, lowGTW group compared with control group and high GTW group (P>0.05). At the laststomached, obvious differences were found between high GTW group and controlgroup (P<0.05).4.The weight of body, testis, liver and their organ coefficients: Whether the laststomached or after caging, during the same period the weight of body, testis and liverof control group almost were the largest, while the testis and liver organ coefficientswere mainly the lowest, these indicators of the blank group compared with CTXgroups, there were remarkable differences in statistics (P<0.05), but there were noremarkable differences compared with GTW groups (P>0.05). Obvious differenceswere found between CTX groups and the corresponding dose GTW groups at the sametime (P<0.05), and there were remarkable changes on those indicators(except the livercoefficient) between low CTX group and high CTX group over the same period(P<0.05), while high GTW group compared with low GTW group on these indicatorsduring the same period, there were no remarkable differences in statistics (P>0.05).5.The average time of pregnancy, the average litter size and conception rate: From theaverage time of pregnancy, low CTX group was the longest, secondly, high GTWgroup, the blank group was the shortest, low GTW group was very close to controlgroup, and there were no significant differences (P>0.05), low CTX group and high GTW group respectively compared with the control group and low GTW group, therewere significant differences (P<0.05); order in terms of the average litter size wasfollows:the control group>low GTW group>high GTW group>low CTX group, lowCTX group and the other groups were significantly different (P<0.05); from theconception rate, blank group (100%) ranked first, the lowest of low CTX group, was60%, high and low GTW groups respectively were92.86%and96.67%, low CTXgroup and the other groups were significantly different (P<0.05). The rats of highCTX group all were dead, without the corresponding indicators of fertility.Conclusion:1. Clinical dose of CTX can decrease the number of white blood cells in experimentalrats, which recovers after drug discontinuation; the clinical dose GTW has little effecton the number of WBC in rats.2. The clinical dose CTX and GTW has no obvious impact on ALT and AST ofexperimental rats.3. The conventional dose GTW has no significant effect on fertility of experimentalrat, double dose GTW has certain influence on fertility in the rats after8weeks oftreatment, but the effect is reversible; double dose GTW on fertility of rats issignificantly less than that of low dose CTX. |
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